Impact of tillage system on arbuscular mycorrhiza fungal communities in the soil under Mediterranean conditions

被引:113
作者
Brito, Isabel [1 ]
Goss, Michael J. [2 ]
de Carvalho, Mario [1 ]
Chatagnier, Odile [3 ]
van Tuinen, Diederik [3 ]
机构
[1] Univ Evora, ICAAM, P-7002554 Evora, Portugal
[2] Univ Guelph, Kemptville, ON K0G 1J0, Canada
[3] Univ Bourgogne, PME INRA CMSE, CNRS 5184, UMR INRA 1084, F-21065 Dijon, France
关键词
Arbuscular mycorrhiza (AM) fungi; Genetic diversity; Soil tillage; Large ribosomal subunit (LSU rDNA); GLOMUS-INTRARADICES; ROOT COLONIZATION; DIVERSITY; DISTURBANCE; PLANT; L;
D O I
10.1016/j.still.2012.01.012
中图分类号
S15 [土壤学];
学科分类号
090301 [土壤学];
摘要
A more diverse arbuscular mycorrhiza (AM) fungal community should be more versatile and resilient to variation in environmental conditions over space and time. To evaluate the effect of no-till and conventional tillage systems, AM fungal diversity was assessed as part of a long term field experiment by sequencing of DNA, extracted from soil, that encoded the large ribosomal sub-unit and was obtained by nested-PCR. In comparison with no-till, conventional tillage decreased AM fungal diversity by 40%. Differences between treatments in the frequency of the operational taxonomic units (OTUs) present in soil, confirm that AM fungi are differently vulnerable to soil disturbance. (C) 2012 Elsevier B.V. All rights reserved.
引用
收藏
页码:63 / 67
页数:5
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