DNA double-strand breaks and homology search:: inferences from a species with incomplete pairing and synapsis

被引:25
作者
Calvente, A
Viera, A
Page, J
Parra, MT
Gómez, R
Suja, JA
Rufas, JS [1 ]
Santos, JL
机构
[1] Univ Autonoma Madrid, Fac Ciencias, Dept Biol, E-28049 Madrid, Spain
[2] Univ Complutense Madrid, Fac Biol, Dept Genet, E-28040 Madrid, Spain
关键词
double-strand breaks; synapsis; recombination; gamma-H2AX; meiosis; RAD51;
D O I
10.1242/jcs.02391
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The relationship between meiotic recombination events and different patterns of pairing and synapsis has been analysed in prophase I spermatocytes of the grasshopper Stethophyma grossum, which exhibit very unusual meiotic characteristics, namely (1) the three shortest bivalents achieve full synapsis and do not show chiasma localisation; (2) the remaining eight bivalents show restricted synapsis and proximal chiasma localisation, and (3) the X chromosome remains unsynapsed. We have studied by means of immunofluorescence the localisation of the phosphorylated histone H2AX (gamma-H2AX), which marks the sites of double-strand breaks; the SMC3 cohesin subunit, which is thought to have a close relationship to the development of the axial element (a synaptonemal complex component); and the recombinase RAD51. We observed a marked nuclear polarization of both the maturation of SMC3 cohesin axis and the ulterior appearance of gamma-H2AX and RAD51 foci, these being exclusively restricted to those chromosomal regions that first form cohesin axis stretches. This polarised distribution of recombination events is maintained throughout prophase I over those autosomal regions that are undergoing, or about to undergo, synapsis. We propose that the restricted distribution of recombination events along the chromosomal axes in the spermatocytes is responsible for the incomplete presynaptic homologous alignment and, hence, for the partial synaptonemal complex formation displayed by most bivalents.
引用
收藏
页码:2957 / 2963
页数:7
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