Functional characterization and genetic mapping of alk8

被引：35

作者：

Payne, TL

Postlethwait, JH

Yelick, PC

机构：

[1] Forsyth Inst, Dept Cytokine Biol, Boston, MA 02115 USA

[2] Forsyth Inst, Harvard Forsyth Dept Oral Biol, Boston, MA 02115 USA

[3] Univ Oregon, Inst Neurosci, Eugene, OR 97403 USA

来源：

MECHANISMS OF DEVELOPMENT | 2001年 / 100卷 / 02期

关键词：

zebrafish; transforming growth factor beta family member signaling; activin-like-kinase receptors; bone morphogenetic protein signaling; dorsoventral patterning;

D O I：

10.1016/S0925-4773(00)00541-4

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The novel type I TGF beta family member receptor alk8 is expressed both maternally and zygotically. Functional characterization of alk8 was performed using microinjection studies of constitutively active (CPI), kinase modified/dominant negative (DN), and truncated alk8 mRNAs. CA Alk8 expression produces ventralized embryos while DN Alk8 expression results in dorsalized phenotypes. Truncated alk8 expressing embryos display a subtle dorsalized phenotype closely resembling that of the identified zebrafish dorsalized mutant, lost-a-fin (laf). Single-strand conformation polymorphism (SSCP) analysis was used to map alk8 to zebrafish LG02 in a region demonstrating significant conserved synteny to Hsa2, and which contains the human alk2 gene, ACVRI. Altogether, these functional, gene mapping and phylogenetic analyses suggest that alk8 may be the zebrafish orthologue to human ACVRI (alk2), and therefore extend previous studies of Alk2 conducted in Xenopus. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：275 / 289

页数：15

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