The hermes transposon of Musca domestica is an efficient tool for the mutagenesis of Schizosaccharomyces pombe

被引：26

作者：

Evertts, Adam G. ^{[1
]}

Plymire, Christopher ^{[1
]}

Craig, Nancy L. ^{[2
,3
]}

Levin, Henry L. ^{[1
]}

机构：

[1] NICHHD, Sect Eukaryot Transposable Elements, Lab Gene Regulat & Dev, NIH, Bethesda, MD 20892 USA

[2] Howard Hughes Med Inst, Dept Mol Biol, Baltimore, MD 21205 USA

[3] Johns Hopkins Univ, Sch Med, Baltimore, MD 21205 USA

来源：

GENETICS | 2007年 / 177卷 / 04期

关键词：

D O I：

10.1534/genetics.107.081075

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Currently, no transposon-based method for the mutagenesis of Schizosaccharomyces pombe exists. We have developed such a system based on teh introduction of the hermes transposon from the housefly into S. pombe. This system efficiently disrupts openreading frames and allows the insertion sites to be readily identified.

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页码：2519 / 2523

页数：5

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