Circulating Red Cell-derived Microparticles in Human Malaria

被引:124
作者
Nantakomol, Duangdao [2 ]
Dondorp, Arjen M. [5 ]
Krudsood, Srivicha
Udomsangpetch, Rachanee [3 ]
Pattanapanyasat, Kovit [4 ]
Combes, Valery [6 ]
Grau, Georges E. [6 ]
White, Nicholas J. [5 ]
Viriyavejakul, Parnpen
Day, Nicholas P. J. [5 ]
Chotivanich, Kesinee [1 ]
机构
[1] Mahidol Univ, Fac Trop Med, Dept Clin Trop Med, Bangkok 10400, Thailand
[2] Chulalongkorn Univ, Fac Allied Hlth Sci, Dept Clin Microscopy, Bangkok, Thailand
[3] Mahidol Univ, Fac Sci, Dept Pathobiol, Bangkok 10400, Thailand
[4] Mahidol Univ, Fac Med, Siriraj Hosp, Bangkok 10700, Thailand
[5] Churchill Hosp, Ctr Trop Med, Oxford, England
[6] Univ Sydney, Fac Med, Dept Pathol, Vasc Immunol Unit, Camperdown, NSW, Australia
基金
英国惠康基金;
关键词
SEVERE FALCIPARUM-MALARIA; ENDOTHELIAL MICROPARTICLES; BLOOD-CELLS; FLOW; PHOSPHATIDYLSERINE; ERYTHROCYTES; QUANTITATION; RECOGNITION; EXPRESSION; RELEASE;
D O I
10.1093/infdis/jiq104
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
In patients with falciparum malaria, plasma concentrations of cell-derived microparticles correlate with disease severity. Using flow cytometry, we quantified red blood cell-derived microparticles (RMPs) in patients with malaria and identified the source and the factors associated with production. RMP concentrations were increased in patients with Plasmodium falciparum (n = 29; median, 457 RMPs/mu L [range, 13-4,342 RMPs/mu L]), Plasmodium vivax (n = 5; median, 409 RMPs/mu L [range, 281-503/mu L]), and Plasmodium malariae (n = 2; median, 163 RMPs/mu L [range, 127-200 RMPs/mu L]) compared with those in healthy subjects (n = 11; median, 8 RMPs/mu L [range, 3-166 RMPs/mu L]; P = .01). RMP concentrations were highest in patients with severe falciparum malaria (P = .01). Parasitized red cells produced > 10 times more RMPs than did unparasitized cells, but the overall majority of RMPs still derived from uninfected red blood cells (URBCs). In cultures, RMP production increased as the parasites matured. Hemin and parasite products induced RMP production in URBCs, which was inhibited by N-acetylcysteine, suggesting heme-mediated oxidative stress as a pathway for the generation of RMPs.
引用
收藏
页码:700 / 706
页数:7
相关论文
共 28 条
[1]  
Beales PF, 2000, T ROY SOC TROP MED H, V94, pS1
[2]   STUDIES OF FACTORS REGULATING THE AGING OF HUMAN-ERYTHROCYTES .3. METABOLISM AND FATE OF ERYTHROCYTIC VESICLES [J].
BOCCI, V ;
PESSINA, GP ;
PAULESU, L .
INTERNATIONAL JOURNAL OF BIOCHEMISTRY, 1980, 11 (02) :139-142
[3]   Cell vesiculation and immunopathology: implications in cerebral malaria [J].
Coltel, Nicolas ;
Combes, Valery ;
Wassmer, Samuel C. ;
Chimini, Giovanna ;
Grau, Georges E. .
MICROBES AND INFECTION, 2006, 8 (08) :2305-2316
[4]   In vitro generation of endothelial microparticles and possible prothrombotic activity in patients with lupus anticoagulant [J].
Combes, V ;
Simon, AC ;
Grau, GE ;
Arnoux, D ;
Camoin, L ;
Sabatier, F ;
Mutin, M ;
Sanmarco, M ;
Sampol, J ;
Dignat-George, F .
JOURNAL OF CLINICAL INVESTIGATION, 1999, 104 (01) :93-102
[5]   Circulating endothelial microparticles in Malawian children with severe falciparum malaria complicated with coma [J].
Combes, V ;
Taylor, TE ;
Juhan-Vague, I ;
Mège, JL ;
Mwenechanya, J ;
Tembo, M ;
Grau, GE ;
Molyneux, ME .
JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 2004, 291 (21) :2542-2544
[6]   The release of microparticles by apoptotic cells and their effects on macrophages [J].
Distler, JHW ;
Huber, LC ;
Hueber, AJ ;
Reich, CF ;
Gay, S ;
Distler, O ;
Pisetsky, DS .
APOPTOSIS, 2005, 10 (04) :731-741
[7]   Reduced microcirculatory flow in severe falciparum malaria: pathophysiology and electron-microscopic pathology [J].
Dondorp, AM ;
Pongponratn, E ;
White, NJ .
ACTA TROPICA, 2004, 89 (03) :309-317
[8]   Cytoadherence of malaria-infected red blood cells involves exposure of phosphatidylserine [J].
Eda, S ;
Sherman, IW .
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, 2002, 12 (5-6) :373-384
[9]  
JY W, 1992, J LAB CLIN MED, V119, P334
[10]   SYNCHRONIZATION OF PLASMODIUM-FALCIPARUM ERYTHROCYTIC STAGES IN CULTURE [J].
LAMBROS, C ;
VANDERBERG, JP .
JOURNAL OF PARASITOLOGY, 1979, 65 (03) :418-420