Serial changes in cerebral blood flow and flow-metabolism uncoupling in primates with acute thromboembolic stroke

被引:34
作者
Kuge, Y
Yokota, C
Tagaya, M
Hasegawa, Y
Nishimura, A
Kito, G
Tamaki, N
Hashimoto, N
Yamaguchi, T
Minematsu, K
机构
[1] Hokkaido Univ, Grad Sch Med, Dept Tracer Kinet, Kita Ku, Sapporo, Hokkaido 0608638, Japan
[2] Hokkaido Univ, Grad Sch Med, Dept Nucl Med, Kita Ku, Sapporo, Hokkaido 0608638, Japan
[3] Inst Biofunct Res Ltd, Osaka, Japan
[4] Natl Cardiovasc Ctr, Cerebrovasc Lab, Res Inst, Osaka, Japan
[5] Natl Cardiovasc Ctr, Dept Med, Cerebrovasc Div, Osaka, Japan
[6] Shin Nippon Biomed Labs Ltd, Kagoshima, Japan
关键词
thromboembolic stroke; cerebral blood flow; cerebral metabolic rate of glucose; positron emission tomography; monkey;
D O I
10.1097/00004647-200103000-00003
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The authors recently developed a primate thromboembolic stroke model. To characterize the primate model, the authors determined serial changes in cerebral blood flow (CBF) and the relation between CBF and cerebral metabolic rate of glucose (CMRglc) using high-resolution positron emission tomography. Thromboembolic stroke was produced in male cynomolgus monkeys (n = 4). Acute obstruction of the left middle cerebral artery was achieved by injecting an autologous blood clot into the left internal carotid artery. Cerebral blood flow was measured with [O-15]H2O before and 1, 2, 4, 6, and 24 hours after embolization. CMRglc was measured with 2-[F-18]fluoro-2-deoxy-D-glucose ([F-18]FDG) 24 hours after embolization. Lesion size and location 24 hours after embolization was determined by the 2,3,5-triphenyl-tetrazolium chloride (TTC) staining method. The results are summarized as follows: (1) 1 hour after embolization, CBF in the temporal cortex and the basal ganglia decreased to < 40% of the contralateral values. Ln these regions, regarded as an ischemic core, CBF decreased further with time and CMRglc at 24 hours also decreased. Infarcted lesions as indicated by being unstained with TTC were consistently observed in these regions. (2) In the parietal cortex and several regions surrounding the ischemic core, CBF was >40% of the contralateral values 1 hour after embolization and recovered gradually with time (ischemic penumbra). In these regions, CMRglc at 24 hours increased compared with that in the contralateral regions, indicating an uncoupling of CBF and CMRglc. No obvious TTC-unstained lesions were detected in these regions. The authors demonstrated a gradual recovery of reduced CBF, an elevated CMRglc and a CBF-CMRglc uncoupling in the penumbra regions of the primate model. Positron emission tomography investigations using this model will provide better understanding of the pathophysiology of thromboembolic stroke in humans.
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收藏
页码:202 / 210
页数:9
相关论文
共 40 条
[1]   3-DIMENSIONAL IMAGE-ANALYSIS OF BRAIN GLUCOSE-METABOLISM BLOOD-FLOW UNCOUPLING AND ITS ELECTROPHYSIOLOGICAL CORRELATES IN THE ACUTE ISCHEMIC PENUMBRA FOLLOWING MIDDLE CEREBRAL-ARTERY OCCLUSION [J].
BACK, T ;
ZHAO, WZ ;
GINSBERG, MD .
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM, 1995, 15 (04) :566-577
[2]  
Baron JC, 1996, NEUROSURG CLIN N AM, V7, P653
[3]   ARTIFICIAL EMBOLIZATION OF MIDDLE CEREBRAL-ARTERY IN PRIMATES - DESCRIPTION OF AN EXPERIMENTAL MODEL WITH EXTRACRANIAL TECHNIQUE [J].
BREMER, AM ;
WATANABE, O ;
BOURKE, RS .
STROKE, 1975, 6 (04) :387-390
[4]  
CHOPP M, 1993, CURR OPIN NEUROL NEU, V6, P6
[5]   VARIABILITY AND REVERSIBILITY OF FOCAL CEREBRAL-ISCHEMIA IN UNANESTHETIZED MONKEYS [J].
CROWELL, RM ;
MARCOUX, FW ;
DEGIROLAMI, U .
NEUROLOGY, 1981, 31 (10) :1295-1302
[6]  
DELAY G, 1988, J CEREB BLOOD F MET, V8, P539
[7]   EXPERIMENTAL ACUTE THROMBOTIC STROKE IN BABOONS [J].
DELZOPPO, GJ ;
COPELAND, BR ;
HARKER, LA ;
WALTZ, TA ;
ZYROFF, J ;
HANSON, SR ;
BATTENBERG, E .
STROKE, 1986, 17 (06) :1254-1265
[8]   Recommendations for standards regarding preclinical neuroprotective and restorative drug development [J].
Feinklestein, SP ;
Fisher, M ;
Furland, AJ ;
Goldstein, LB ;
Gorelick, PB ;
Kaste, M ;
Lees, KR ;
Traystman, RJ ;
Albers, GW ;
Anwer, UE ;
Ashwood, T ;
Barone, FC ;
Basta, SL ;
Bogousslavsky, J ;
Buchan, AM ;
Cady, WJ ;
Chan, PH ;
Clemens, JA ;
Cox, BF ;
Craddock, RE ;
Cramer, SC ;
del Zoppo, GJ ;
Dielrich, WD ;
Elliott, P ;
Faden, AI ;
Feuerstein, GZ ;
Ginsberg, MD ;
Gold, M ;
Greene, WL ;
Hall, ED ;
Hsu, CY ;
Hunter, AJ ;
Lai, M ;
Lesko, LM ;
Levy, DE ;
Li, FH ;
Locke, KW ;
Lodge, D ;
Lowe, D ;
Marcoux, FW ;
McCulloch, J ;
McDermott, J ;
Meibach, R ;
Messersmith, EK ;
Moseley, M ;
Moskowitz, MA ;
Mueller, AL ;
Munro, F ;
Nudo, RJ ;
Oeda, J .
STROKE, 1999, 30 (12) :2752-2758
[9]   LOCAL GLUCOSE-UTILIZATION IN ACUTE FOCAL CEREBRAL ISCHEMIA - LOCAL DYSMETABOLISM AND DIASCHISIS [J].
GINSBERG, MD ;
REIVICH, M ;
GIANDOMENICO, A ;
GREENBERG, JH .
NEUROLOGY, 1977, 27 (11) :1042-1048
[10]   CEREBRAL BLOOD-FLOW, GLUCOSE-UTILIZATION, AND ELECTROCORTICOGRAMS FOLLOWING COMMON CAROTID-ARTERY OCCLUSION IN GERBILS [J].
HASEGAWA, Y ;
CHOKI, J ;
YAMAGUCHI, T .
STROKE, 1990, 21 (01) :112-118