Functional and pathological effects of prolonged hyperoxia in neonatal mice

被引:348
作者
Warner, BB [1 ]
Stuart, LA [1 ]
Papes, RA [1 ]
Wispé, JR [1 ]
机构
[1] Childrens Hosp, Med Ctr, Div Neonatol & Pulm Biol, Cincinnati, OH 45229 USA
关键词
bronchopulmonary dysplasia; lung; cell proliferation; newborn;
D O I
10.1152/ajplung.1998.275.1.L110
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Bronchopulmonary dysplasia (BPD) commonly develops in premature infants. An improved understanding of the pathophysiology of BPD requires better models. In this study, neonatal FVB/N mice were exposed to room air or 85% oxygen for 28 days. Neonatal hyperoxia resulted in decreased alveolar septation, increased terminal air space size, and increased lung fibrosis. These changes were evident after 7 days and more pronounced by 28 days. Decreased alveolarization was preceded by decreased proliferation of lung cells. After 3 days of hyperoxia, cell proliferation was decreased compared with room air littermates. Cell proliferation continued to be decreased in the first 2 wk but normalized by 4 wk. Hyperoxia caused an increased number of inflammatory cells in lung tissue and in lung lavage fluid. Analysis of lung tissue RNA by RT-PCR showed that hyperoxia increased expression of the proinflammatory cytokines interleukin-la: and macrophage inflammatory protein-la. Prolonged neonatal hyperoxia caused functional changes, decreasing lung volume and pulmonary compliance. We conclude that prolonged exposure of neonatal mice to hyperoxia creates a lesion that is very similar to human BPD and suggests that altered cell proliferation may be important in the pathogenesis of chronic neonatal lung disease.
引用
收藏
页码:L110 / L117
页数:8
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