Differential expression analysis by cDNA-AFLP of Saccharum spp. after inoculation with the host pathogen Sporisorium scitamineum

被引：31

作者：

LaO, Maria ^{[1
,2
]}

Arencibia, Ariel D. ^{[1
]}

Carmona, Elva R. ^{[1
]}

Acevedo, Ricardo ^{[1
]}

Rodriguez, Eida ^{[1
]}

Leon, Ondina ^{[2
]}

Santana, Ignacio ^{[1
]}

机构：

[1] Natl Inst Sugarcane Res, Havana 19390, Cuba

[2] Natl Ctr Anim & Plant Hlth, Havana, Cuba

来源：

PLANT CELL REPORTS | 2008年 / 27卷 / 06期

关键词：

Sporisorium scitamineum; Saccharum spp; signaling; cDNA-AFLP;

D O I：

10.1007/s00299-008-0524-y

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A differential expression study on the Saccharum spp.-Sporisorium scitamineum pathogenic interaction was carried out in the susceptible Ja60-5 and the resistant M31/45 genotypes. Using cDNA-AFLP analysis, a total of 64 transcript-derived fragments (TDFs) was found to be differentially expressed, with the majority (67.2%) of the differential TDFs up-regulated in the resistant M31/45 cultivar. The plant response against S. scitamineum infection was complex, representing major genes involved in oxidative burst, defensive response, ethylene and auxins pathways during the first 72 h post-inoculation. Results obtained suggest a key role for genes involved in the oxidative burst and the lignin pathways in the initial sugarcane defense against the S. scitamineum infection.

引用

页码：1103 / 1111

页数：9

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