Determination of free calcium and calcium-containing species in human plasma by capillary electrophoresis-inductively coupled plasma optical emission spectrometry

被引：44

作者：

Deng, Biyang ^{[1
,2
]}

Zhu, Pingchuan ^{[1
]}

Wang, Yingzi ^{[1
]}

Feng, Jinrong ^{[1
]}

Li, Xianfeng ^{[1
]}

Xu, Xiangshu ^{[1
]}

Lu, Hua ^{[2
]}

Xu, Qiumei ^{[1
]}

机构：

[1] Guangxi Normal Univ, Coll Chem & Chem Engn, Guilin 541004, Peoples R China

[2] Guilin Univ Technol, Dept Mat & Chem Engn, Guilin 541004, Peoples R China

来源：

ANALYTICAL CHEMISTRY | 2008年 / 80卷 / 15期

关键词：

D O I：

10.1021/ac800715c

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new method for the determination of free calcium concentration in human plasma was developed by online coupling capillary electrophoresis (CE) with inductively coupled plasma optical emission spectrometry (ICP-OES). Baseline separation of calcium-containing species was achieved by CE-ICP-OES in a 120-cm-long capillary with 100-mu m internal diameter, at 20 kV applied voltage, with a 30 mmol/L Tris-HCl buffer at pH 7.4. A total of eight calcium-containing species were found in human plasma; the concentration of free calcium ion was found to be 41.9 mg/L. The concentrations of calcium for other seven calcium species, estimated from the calibration against Ca2+ standard, were 3.14-15.6 mg/L. The precision (RSD, n = 10) ranged from 1.2 to 2.7% for the migration time and 2.8 to 3.9% for the peak area. The developed method was also applied to analyze plasma samples with recovery ranged from 94.5 to 102% for samples spiked with 40 mg/L free Ca2+ ion.

引用

页码：5721 / 5726

页数：6

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