Activin co-operates with fibroblast growth factor 2 to regulate tyrosine hydroxylase expression in the basal forebrain ventricular zone progenitors

被引:24
作者
Daadi, M
Arcellana-Panlilio, MY
Weiss, S
机构
[1] Univ Calgary, Fac Med, Neurosci Res Grp, Dept Anat, Calgary, AB T2N 4N1, Canada
[2] Univ Calgary, Fac Med, Neurosci Res Grp, Dept Pharmacol & Therapeut, Calgary, AB T2N 4N1, Canada
基金
英国医学研究理事会;
关键词
local cues; TGF beta s; forebrain precursors; plasticity in neurotransmitter phenotype expression; cellular diversity; neurodegenerative diseases;
D O I
10.1016/S0306-4522(98)00102-X
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Activin and its cognate receptors are expressed during embryogenesis in the rapidly dividing cells of the basal forebrain ventricular zone. This finding prompted us to study the role of activin in regulating neurotransmitter phenotype expression and other aspects of the ventricular zone-derived progenitor cell differentiation. although virtually ineffective alone, activin co-operated with fibroblast growth factor 2 to induce a rapid tyrosine hydroxylase-immunoreactivity in cultured ventricular zone progenitors. Northern analysis indicated that the increase in tyrosine hydroxylase-immunoreacrivity was associated with increased tyrosine hydroxylase gene expression. Activin and fibroblast growth factor 2 action was specific to tyrosine hydroxylase, as it did not induce the expression of choline acetyltransferase, nor enhance the expression of glutamate decarboxylase. Cultures treated with the DNA replication marker bromodeoxyuridine revealed that both proliferating ventricular zone progenitors and their post-mitotic progeny were induced to express tyrosine hydroxylase. In these cultures, activin acted to reduce fibroblast growth Factor 2 stimulated mitotic activity. Furthermore, activin permitted neuronal differentiation and survival of the ventricular zone progenitors after three days in vitro. Together these data demonstrate a novel role of activin and fibroblast growth factor 2 in regulating the Fate of the embryonic basal forebrain ventricular zone progenitors. (C) 1998 IBRO. Published by Elsevier Science Ltd.
引用
收藏
页码:867 / 880
页数:14
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