Determining the zone of impact of norovirus contamination in shellfish production areas through microbiological monitoring and hydrographic analysis

被引:13
作者
Campos, Carlos J. A. [1 ]
Goblick, Gregory [2 ]
Lee, Ron [1 ,3 ]
Wittamore, Ken [4 ]
Lees, David N. [1 ]
机构
[1] Ctr Environm Fisheries & Aquaculture Sci Cefas, Weymouth Lab, Barrack Rd, Weymouth DT4 8UB, England
[2] US FDA, 5100 Paint Branch Pkwy, College Pk, MD 20740 USA
[3] MicroSeaSafe, 33 Franklin Rd, Weymouth DT4 0JW, England
[4] Triskel Marine Ltd, 12 St Fimbarrus Rd, Fowey PL23 1JJ, England
关键词
Rhodamine WT; E; coli; Oysters; Mussels; Plume tracking; Estuaries; WASTE-WATER; OUTBREAKS; OYSTERS; SEAFOOD; TRANSPORT; VIRUSES; SEWAGE;
D O I
10.1016/j.watres.2017.08.021
中图分类号
X [环境科学、安全科学];
学科分类号
083001 [环境科学];
摘要
Norovirus (NoV) contamination of filter feeding bivalve shellfish is a well-recognised human health threat when shellfish are grown in sewage polluted waters. To date, the identification of high risk zones around sewage discharges in shellfish production areas (SPAs) has not been based on NoV data. This study utilised molecular methods for NoV analysis, combined with hydrographic studies, to determine the relationship between NoV concentrations in shellfish and sewage effluent dilution. Cages with mussels and oysters were placed at different distances downstream of sewage discharges in two coastal sites in England. The shellfish were tested for concentrations of NoV (genogroups I and II) and E. coli. Drogue tracking and dye tracing studies were conducted to quantify the dispersion and dilution of sewage effluent in the SPAs. Significant negative associations were found between both total concentrations of NoV (GI + GII) and E. coli and sewage effluent dilution in the SPAs. The total NoV concentrations predicted by the model at 300:1,1000:1 and 5000:1 ratios of estuarine water to sewage effluent were 1200; 600; and 200 copies/g, respectively. The estimated area of NoV contamination varied according with local pollution source impacts and hydrographic characteristics. The results help to inform the derivation of sewage discharge buffer zones as a control measure for mitigating risk from human NoV contamination in SPAs. Crown Copyright (C) 2017 Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码:556 / 565
页数:10
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