Carbohydrate expression and modification during keratinocyte differentiation in normal human and reconstructed epidermis

被引：13

作者：

Méhul, B ^{[1
]}

Corre, C ^{[1
]}

Capon, C ^{[1
]}

Bernard, D ^{[1
]}

Schmidt, R ^{[1
]}

机构：

[1] LOreal Rech, Paris, France

来源：

EXPERIMENTAL DERMATOLOGY | 2003年 / 12卷 / 05期

关键词：

carbohydrates; epidermis; glycoconjugates; glycosidases; stratum corneum;

D O I：

10.1034/j.1600-0625.2003.00049.x

中图分类号：

R75 [皮肤病学与性病学];

学科分类号：

100206 ;

摘要：

Using fluorescein isothiocyanate (FITC)-labeled lectins we were able to demonstrate the presence of specific carbohydratemoieties in normal human and reconstructed epidermis. Evidence is provided that in both cases the strongly reduced lectin staining at the level of the stratum corneum is the result of a hindered accessibility of the lectins in this lipid-rich hydrophobic environment. Isolated corneocytes and purified cornified envelopes (CEs) exhibited clearly glycosylated structures reacting with distinct lectins. The presence of glycosidase activity, particularly in the upper layers of the epidermis characterized by an acidic environment (pH 5.5), indicates that modifications of the sugar residues might be important in epidermal homeostasis, barrier behavior and desquamation. Absent or strongly reduced glycosidase activity in the stratum corneum of reconstructed epidermis with an impaired pH gradient could be in part responsible for the reduced barrier function and the lack of desquamation in this model.

引用

页码：537 / 545

页数：9

共 41 条

[1] Purification and characterization of the endoglycosidase heparanase 1 from human plantar stratum corneum:: a key enzyme in epidermal physiology? [J].