Cytokine mRNA profiles in contused spinal cord and axotomized facial nucleus suggest a beneficial role for inflammation and gliosis

被引:315
作者
Streit, WJ [1 ]
Semple-Rowland, SL
Hurley, SD
Miller, RC
Popovich, PG
Stokes, BT
机构
[1] Univ Florida, Coll Med, Dept Neurosci, Gainesville, FL 32610 USA
[2] Univ Florida, Inst Brain, Gainesville, FL 32610 USA
[3] Ohio State Univ, Dept Med Microbiol & Immunol, Columbus, OH 43210 USA
[4] Ohio State Univ, Dept Physiol, Columbus, OH 43210 USA
[5] Ohio State Univ, Coll Med, Spinal Cord Injury Res Ctr, Columbus, OH 43210 USA
关键词
degeneration; regeneration; spinal cord injury; microglia; blood-brain barrier; growth factors;
D O I
10.1006/exnr.1998.6835
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
We have studied temporal mRNA expression patterns for interleukin-1 beta (IL-1 beta), tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6), macrophage colony stimulating factor (M-CSF), and transforming growth factor-beta 1 (TGF-beta 1) in two rat injury paradigms with very different cellular inflammatory reactions: contussion of the spinal cord and axotomy of the facial nerve. Our comparative analyses using semiquantitative reverse transcription polymerase chain reaction (RT-PCR) show an early and robust upregulation of IL-1 beta, TNF-alpha, IL-6, and M-CSF mRNAs in spinal cord after contusion injury. Peak expression of these mRNAs was transient and returned to control levels by 24 h postinjury. In contrast, expression of IL-1 beta and TNF-alpha mRNAs in the axotomized facial nucleus was minimal and delayed, and levels of M-CSF mRNA remained unaltered. Similar to injured spinal cord, the axotomized nucleus showed a dramatic and early upregulation of IL-6 mRNA but unlike spinal cord, IL-6 mRNA levels subsided only gradually. Both injury paradigms showed gradually increasing levels of TGF-beta 1 mRNA which were maximal at 7 days postinjury. RT-PCR analyses were also performed on isolated blood-borne mononuclear cells and neutrophils. The results showed that these cells contain high levels of IL-1 beta and M-CSF mRNAs, moderate levels of TGF-beta and TNF-alpha mRNAs, and minimal levels of IL-6 mRNA The RT-PCR analyses together with histological observations indicate that expression of the proinflammatory cytokines IL-1 beta, TNF-alpha, and IL-6 is short-lived and self-limited after contusion injury, and that it occurs primarily within endogenous glial cells. Transient expression of these molecules likely triggers secondary events which may be beneficial to wound repair and regeneration. (C) 1998 Academic Press.
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收藏
页码:74 / 87
页数:14
相关论文
共 75 条
[1]   INTRACEREBRAL INJECTION OF PROINFLAMMATORY CYTOKINES OR LEUKOCYTE CHEMOTAXINS INDUCES MINIMAL MYELOMONOCYTIC CELL RECRUITMENT TO THE PARENCHYMA OF THE CENTRAL-NERVOUS-SYSTEM [J].
ANDERSSON, PB ;
PERRY, VH ;
GORDON, S .
JOURNAL OF EXPERIMENTAL MEDICINE, 1992, 176 (01) :255-259
[2]   The role of inflammation and cytokines in brain injury [J].
Arvin, B ;
Neville, LF ;
Barone, FC ;
Feuerstein, GZ .
NEUROSCIENCE AND BIOBEHAVIORAL REVIEWS, 1996, 20 (03) :445-452
[3]   PERINEURONAL GLIAL RESPONSES AFTER AXOTOMY OF CENTRAL AND PERIPHERAL AXONS - A COMPARISON [J].
BARRON, KD ;
MARCIANO, FF ;
AMUNDSON, R ;
MANKES, R .
BRAIN RESEARCH, 1990, 523 (02) :219-229
[4]   METHYLPREDNISOLONE INHIBITS EARLY INFLAMMATORY PROCESSES BUT NOT ISCHEMIC CELL-DEATH AFTER EXPERIMENTAL SPINAL-CORD LESION IN THE RAT [J].
BARTHOLDI, D ;
SCHWAB, ME .
BRAIN RESEARCH, 1995, 672 (1-2) :177-186
[5]   Expression of pro-inflammatory cytokine and chemokine mRNA upon experimental spinal cord injury in mouse: An in situ hybridization study [J].
Bartholdi, D ;
Schwab, ME .
EUROPEAN JOURNAL OF NEUROSCIENCE, 1997, 9 (07) :1422-1438
[6]   SPINAL-CORD INJURY PRODUCED BY CONSISTENT MECHANICAL DISPLACEMENT OF THE CORD IN RATS - BEHAVIORAL AND HISTOLOGIC ANALYSIS [J].
BEHRMANN, DL ;
BRESNAHAN, JC ;
BEATTIE, MS ;
SHAH, BR .
JOURNAL OF NEUROTRAUMA, 1992, 9 (03) :197-217
[7]  
Blight AR, 1992, J NEUROTRAUMA, V9, P83
[8]   DISPLACEMENT OF SYNAPTIC TERMINALS FROM REGENERATING MOTONEURONS BY MICROGLIAL CELLS [J].
BLINZING.K ;
KREUTZBE.G .
ZEITSCHRIFT FUR ZELLFORSCHUNG UND MIKROSKOPISCHE ANATOMIE, 1968, 85 (02) :145-&
[9]  
BOYUM A, 1968, SCAND J CLIN LAB INV, VS 21, P77
[10]   JUNCTIONS BETWEEN INTIMATELY APPOSED CELL MEMBRANES IN VERTEBRATE BRAIN [J].
BRIGHTMA.MW ;
REESE, TS .
JOURNAL OF CELL BIOLOGY, 1969, 40 (03) :648-+