The action site of carbon dioxide in relation to inhibition of ethylene production in tomato fruit

被引:30
作者
de Wild, HPJ [1 ]
Balk, PA [1 ]
Fernandes, ECA [1 ]
Peppelenbos, HW [1 ]
机构
[1] Agrotechnol & Food Innovat, NL-6700 AA Wageningen, Netherlands
关键词
ACC; ethylene; 1-MCP; mRNA transcripts; tomato;
D O I
10.1016/j.postharvbio.2005.02.004
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
High CO2 can inhibit ethylene production of various fruit. A high level Of CO2 (20 kPa) was applied to tomato fruit (Lycopersicon esculentum Mill. cv Aromata) at I VC for 5 days. To investigate the primary action Site Of CO2, we used tomato fruit at a ripening stage where feedback regulation of ethylene production was of limited importance. Feedback reactions were further prevented by a treatment with I-methylcyclopropene (1-MCP) before exposure to high CO2. Tomatoes with and without 1-MCP pre-treatment were exposed to 0 or 20 kPa CO2. Ethylene production, 1-aminocyclopropane-1-carboxylate (ACC) content and ACC oxidase mRNA abundance were measured after 1, 2 and 5 days exposure to 0 or 20 kPa CO2. High CO2-affected LE-ACO1, LE-ACO3 and LE-ACO4 transcripts differently. Several observations show that high CO2 did not affect the ethylene receptor: (1) CO2 had a much earlier and much stronger inhibitory effect on ethylene production than 1-MCP; (2) CO2 prevented while 1-MCP stimulated ACC accumulation; (3) CO2 prevented the 1-MCP induced decrease of LE-ACO1 abundance, and inhibited the 1-MCP induced decrease of LE-ACO3 abundance. Inhibition of ethylene production together with prevention of ACC accumulation by CO2, both in fruit with and without 1-MCP pre-treatment, points to inhibition at a site before the conversion of ACC to ethylene. (c) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码:273 / 280
页数:8
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