Differentiation of chicken umbilical cord mesenchymal stem cells into beta-like pancreatic islet cells

被引：19

作者：

Bai, Chunyu ^{[1
,2
]}

Gao, Yuhua ^{[1
,2
]}

Li, Qian ^{[1
,2
]}

Feng, Yuan ^{[1
]}

Yu, Yanze ^{[1
]}

Meng, Gentong ^{[1
]}

Zhang, Minghai ^{[1
]}

Guan, Weijun ^{[2
]}

机构：

[1] Northeast Forestry Univ, Coll Wildlife Resources, Harbin 150040, Peoples R China

[2] Chinese Acad Agr Sci, Inst Anim Sci, Beijing 100193, Peoples R China

来源：

ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY | 2015年 / 43卷 / 02期

关键词：

chicken; islet cells; mesenchymal stem cells; umbilical cord; INSULIN-PRODUCING CELLS; RETINOIC ACID; PROGENITOR CELLS; BONE-MARROW; SECRETING CELLS; ENDOCRINE-CELLS; IN-VITRO; GENERATION; EXPANSION; ENDODERM;

D O I：

10.3109/21691401.2013.864662

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 090105 [作物生产系统与生态工程];

摘要：

In this study, we explored the possibility of using in vitro differentiation to create functional beta-like islet cells from chicken umbilical cord mesenchymal stem cells (UCMSCs). Passaged UCMSCs were induced to differentiate into pancreatic beta-like islet cells. Differentiated cells were observed through dithizone staining, and Pdx1 and insulin expressed in differentiated cells were detected with immunofluorescence. Insulin and C-peptide production from differentiated cells were analyzed using ELISA and western blotting. Differentiated cells were found to not only express Pdx1, insulin, and C-peptide, but also to display a glucose-responsive secretion of these hormones.

引用

页码：106 / 111

页数：6

共 26 条

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Generation of insulin-secreting cells from human embryonic stem cells [J].