The stabilization of housekeeping transcripts in Trypanosoma cruzi epimastigotes evidences a global regulation of RNA decay during stationary phase

被引：10

作者：

Cevallos, AM ^{[1
]}

Pérez-Escobar, M ^{[1
]}

Espinosa, N ^{[1
]}

Herrera, J ^{[1
]}

López-Villaseñor, I ^{[1
]}

Hernández, R ^{[1
]}

机构：

[1] Univ Nacl Autonoma Mexico, Inst Invest Biomed, Dept Mol Biol & Biotecnol, Mexico City 04510, DF, Mexico

来源：

FEMS MICROBIOLOGY LETTERS | 2005年 / 246卷 / 02期

关键词：

protozoa; kinetoplastid; RNA stability; gene expression;

D O I：

10.1016/j.femsle.2005.04.017

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The relative steady state concentration of mRNAs of four housekeeping single-copy type Trypanosoma cruzi genes (actin, triosephosphate isomerase, trypanothion reductase and the ribosomal protein S4) was analyzed throughout the growth curve. A distinguishable pattern was observed with maximal levels occurring at the logarithmic phase of growth and minimum levels occurring at the stationary phase. The half-lives of all analyzed messenger RNAs, and also of three molecular species of immature ribosomal RNAs were increased in cells isolated from stationary phase. These results suggest the occurrence of a novel global regulation mechanism that might protect transcripts from degradation in stationary epimastigotes, probably as a strategy to perpetuate through this quiescent stage. (c) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：259 / 264

页数：6

共 19 条

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