Effects of low O2 and ageing on spindles and chromosomes in mouse oocytes from pre-antral follicle culture

被引:88
作者
Hu, Y
Betzendahl, I
Cortvrindt, R
Smitz, J
Eichenlaub-Ritter, U [1 ]
机构
[1] Univ Hosp Brussels, Ctr Reprod Med, Follicle Biol Unit, Brussels, Belgium
[2] Free Univ Brussels, Sch Med, Brussels, Belgium
[3] Univ Bielefeld, Fac Biol, Inst Gentechnol Microbiol, D-4800 Bielefeld, Germany
关键词
ageing; oocyte; oxygen; pre-antral follicle culture; spindle;
D O I
10.1093/humrep/16.4.737
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
To assess their quality, spindles were analysed in mouse oocytes from pre-antral follicle culture. High or low oxygen tension was present during the last 16 or 20 h post human chorionic gonadotrophin (HCG)/epidermal growth factor (EGF) addition. Most oocytes from pre-antral follicle culture possessed typical anastral spindles with flat poles resembling those of ovulated, in-vivo-matured oocytes of sexually mature mice, while denuded oocytes in-vitro matured to metaphase II (MII) formed significantly longer, slender spindles with pointed, narrow poles. Spindles in oocytes from follicle culture were only slightly shorter and less compact at the equator as compared with those of oocytes matured in vivo. Chromosomes were well aligned at the equator in MII oocytes obtained from follicle culture with high oxygen. Maturation rate was significantly reduced by lowering oxygen tension to 5% O-2. Prolonged culture and the presence of only 5% O-2 dramatically increased the percentage of MII oocytes with unaligned chromosomes. These observations indicate that sufficient oxygen supply and time of retrieval after initiation of resumption of maturation by HCG as well as the microenvironment and cell-cell interactions between oocytes and their somatic compartment are critical in affecting the oocyte's capacity to mature to MII, to form a functional spindle, and to align chromosomes correctly.
引用
收藏
页码:737 / 748
页数:12
相关论文
共 61 条
[1]   Pilot study of isolated early human follicles cultured in collagen gels for 24 hours [J].
Abir, R ;
Roizman, P ;
Fisch, B ;
Nitke, S ;
Okon, E ;
Orvieto, R ;
Ben Rafael, Z .
HUMAN REPRODUCTION, 1999, 14 (05) :1299-1301
[2]   Preservation of fertility in women undergoing chemotherapy: Current approach and future prospects [J].
Abir, R ;
Fisch, B ;
Raz, A ;
Nitke, S ;
Ben-Rafael, Z .
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS, 1998, 15 (08) :469-477
[3]  
ANDERSON JE, 1981, AM LAB, V13, P21
[4]   First-meiotic-division nondisjunction in human oocytes [J].
Angell, R .
AMERICAN JOURNAL OF HUMAN GENETICS, 1997, 61 (01) :23-32
[5]  
Bahadur G, 1996, HUM REPROD, V11, P2215
[6]   Epigenetic modifications necessary for normal development are established during oocyte growth in mice [J].
Bao, SQ ;
Obata, Y ;
Carroll, J ;
Domeki, I ;
Kono, T .
BIOLOGY OF REPRODUCTION, 2000, 62 (03) :616-621
[7]  
Battaglia DE, 1996, HUM REPROD, V11, P2217
[8]   Preservation of fertility and ovarian function and minimizing chemotherapy-induced gonadotoxicity in young women [J].
Blumenfeld, Z ;
Avivi, I ;
Ritter, M ;
Rowe, JM .
JOURNAL OF THE SOCIETY FOR GYNECOLOGIC INVESTIGATION, 1999, 6 (05) :229-239
[9]   CHARACTERIZATION OF FOLLICULAR ENERGY-METABOLISM [J].
BOLAND, NI ;
HUMPHERSON, PG ;
LEESE, HJ ;
GOSDEN, RG .
HUMAN REPRODUCTION, 1994, 9 (04) :604-609
[10]   Transferrin in the developing ovarian follicle: evidence for de-novo expression by granulosa cells [J].
Briggs, DA ;
Sharp, DJ ;
Miller, D ;
Gosden, RG .
MOLECULAR HUMAN REPRODUCTION, 1999, 5 (12) :1107-1114