High expression of leptin by human bone marrow adipocytes in primary culture

被引:161
作者
Laharrague, P
Larrouy, D
Fontanilles, AM
Truel, N
Campfield, A
Tenenbaum, R
Galitzky, J
Corberand, JX
Pénicaud, L
Casteilla, L
机构
[1] Fac Med, IFR L Bugnard, CNRS, UPRESA 5018, Toulouse, France
[2] Fac Med, IFR L Bugnard, INSERM, U317, Toulouse, France
[3] Hoffmann La Roche Inc, Nutley, NJ 07110 USA
关键词
adipose tissue; ob; corticosteroids; hematopoiesis;
D O I
10.1096/fasebj.12.9.747
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Adipocytes participate in the microenvironment of the bone marrow (BM), but their exact role remains to be determined. It has recently been shown that leptin, a hormone secreted from extramedullary adipocytes, could be involved in hematopoiesis. Therefore we have developed a primary culture system of human BM adipocytes to characterize their differentiation and determine whether leptin is also secreted from these adipocytes. BM cells were cultured with fetal calf and horse sera. In the presence of dexamethasone, cells with vesicles containing lipids appeared within 15 days. They expressed glycerol phosphate dehydrogenase activity and a lipolytic activity in response to isoproterenol, but expressed neither the adrenergic beta 3 receptor nor the mitochondrial uncoupling protein UCP1. The addition of insulin alone to the culture media did not promote adipocyte differentiation. Leptin was expressed and secreted at high levels during adipocyte differentiation. Acute exposure of differentiated adipocytes to insulin had little effect on leptin expression whereas forskolin strongly inhibited it. These results show that although human BM adipocytes differ from extramedullary adipose tissues in their sensitivity to different effecters, they are a secondary source of leptin production. They suggest that BM adipocytes could contribute to hematopoiesis via the secretion of leptin in the vicinity of hematopoietic stem cells.
引用
收藏
页码:747 / 752
页数:6
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