Spectral editing in 13C MAS NMR under moderately fast spinning conditions

被引:20
作者
De Vita, E [1 ]
Frydman, L [1 ]
机构
[1] Univ Illinois, Dept Chem MC 111, Chicago, IL 60607 USA
基金
美国国家科学基金会;
关键词
solid-state NMR; magic-angle spinning; spectral assignment techniques; dipolar couplings; polarization transfer;
D O I
10.1006/jmre.2000.2255
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Novel procedures for the spectral assignment of peaks in high-resolution solid-state C-13 NMR are discussed and demonstrated. These methods are based on the observation that at moderate and already widely available rates of magic-angle spinning (10-14 kHz MAS), CH and CH, moieties behave to a large extent as if they were effectively isolated from the surrounding proton reservoir. Dipolar-based analogs of editing techniques that are commonly used in liquid-state NMR such as APT and INEPT can then be derived, while avoiding the need for periods of homonuclear H-1-H-1 multipulse decoupling, The resulting experiments end up being very simple, essentially tuning-free, and capable of establishing unambiguous distinctions among CH, CH2, and -C-/-CH3 carbon sires. The principles underlying such sequences were explored using both numerical calculations and experimental measurements, and once validated their editing applications were illustrated on a number of compounds. (C) 2001 Academic Press.
引用
收藏
页码:327 / 337
页数:11
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