A comparison of three rapid and accurate bioluminescent antibiotic susceptibility tests

被引：8

作者：

Lafond, Mickael ^{[2
]}

Vidal, Nicolas ^{[2
]}

Letourneux, Yves ^{[1
]}

Brunel, Jean Michel ^{[1
]}

机构：

[1] Univ Aix Marseille 2, Fac Med, URMITE, UMR 6236 CNRS, F-13385 Marseille 05, France

[2] Univ Paul Cezanne, UPCAM iSm2, Case 342, F-13397 Marseille 13, France

来源：

JOURNAL OF PHARMACOLOGICAL AND TOXICOLOGICAL METHODS | 2010年 / 61卷 / 01期

关键词：

Bioluminescence; Antibiotic susceptibility; Quinone:NAD(P)H oxydoreductase; CHEMILUMINESCENT ASSAY; ATP-BIOLUMINESCENCE;

D O I：

10.1016/j.vascn.2009.10.004

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Introduction: Rapid determination of microorganisms' antibiotic susceptibility remains a great challenge for the clinical diagnosis of infectious diseases. In this study, we compare three rapid bioluminescent antibiotic susceptibility tests with the conventional normalised microdilution method. Methods: The susceptibility of two bacterial and two fungal strains to a 6 h incubation with one of eight standard antibiotics was investigated by the ATP, AK and QNO bioluminescent methods. Results: Three rapid and accurate antibiotic susceptibility testing were investigated performing ATP, AK and QNO bioluminescent assays. All the results were in accordance with those obtained using a standard microdilution assay. All these methods were sensitive and faster than the previously well-known ones. Discussion: The development of bioluminescent determination methods could lead to accurate and useful antibiotic susceptibility testing. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：16 / 19

页数：4

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