Optimization of ferrofluids and protocols for the enrichment of breast tumor cells in blood

被引：93

作者：

Liberti, PA ^{[1
]}

Rao, CG ^{[1
]}

Terstappen, LWMM ^{[1
]}

机构：

[1] Immunicon Corp, Huntingdon Valley, PA 19006 USA

来源：

JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS | 2001年 / 225卷 / 1-2期

关键词：

tumor cells; breast cancer; ferrofluids; magnetic loading; blood purification; rare cell extraction; non-specific binding;

D O I：

10.1016/S0304-8853(00)01254-3

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

Immune-magnetic selection of rare cells in blood has been optimized by increasing the magnetic loading of target cells and reducing the non-specific binding to non-target cells. Recovery of tumor cells spiked into normal blood ranged from 60 to 90% and is linear over a range of 1-200cells/ml. Tumor cells were found in peripheral blood of breast cancer patients and their numbers increase with disease progression. (C) 2001 Published by Elsevier Science B.V.

引用

页码：301 / 307

页数：7

共 12 条

[1]

Ashworth T., 1869, AUST MED J, V14, P146

[2] Cytokeratin-positive cells in the bone marrow and survival of patients with stage I, II, or III breast cancer. [J].

Braun, S ;

Pantel, K ;

Muller, P ;

Janni, W ;

Hepp, F ;

Kentenich, CRM ;

Gastroph, S ;

Wischnik, A ;

Dimpfl, T ;

Kindermann, G ;

Riethmuller, G ;

Schlimok, G .

NEW ENGLAND JOURNAL OF MEDICINE, 2000, 342 (08) :525-533

[3] Lack of effect of adjuvant chemotherapy on the elimination of single dormant tumor cells in bone marrow of high-risk breast cancer patients [J].

Braun, S ;

Kentenich, C ;

Janni, W ;

Hepp, F ;

de Waal, J ;

Willgeroth, F ;

Sommer, H ;

Pantel, K .

JOURNAL OF CLINICAL ONCOLOGY, 2000, 18 (01) :80-86

[4]

BRUGGER W, 1994, BLOOD, V83, P636

[5]

Liberti PA, 1996, NATO ASI 3 HIGH TECH, V12, P777

[6]

LIBERTI PA, 1991, ACS SYM SER, V464, P268

[7]

Naume B, 1998, INT J CANCER, V78, P556

[8] Immunomagnetic techniques for the enrichment and detection of isolated breast carcinoma cells in bone marrow and peripheral blood [J].

Naume, B ;

Borgen, E ;

Beiske, K ;

Herstad, TK ;

Ravnas, G ;

Renolen, A ;

Trachsel, S ;

ThraneSteen, K ;

Funderud, S ;

Kvalheim, G .

JOURNAL OF HEMATOTHERAPY, 1997, 6 (02) :103-114

[9] Detection and characterization of carcinoma cells in the blood [J].

Racila, E ;

Euhus, D ;

Weiss, AJ ;

Rao, C ;

McConnell, J ;

Terstappen, LWMM ;

Uhr, JW .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (08) :4589-4594

[10]

ROSS AA, 1993, BLOOD, V82, P2605

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