A design of fluorescent probes for superoxide based on a nonredox mechanism

被引：176

作者：

Maeda, H ^{[1
]}

Yamamoto, K ^{[1
]}

Nomura, Y ^{[1
]}

Kohno, I ^{[1
]}

Hafsi, L ^{[1
]}

Ueda, N ^{[1
]}

Yoshida, S ^{[1
]}

Fukuda, M ^{[1
]}

Fukuyasu, Y ^{[1
]}

Yamauchi, Y ^{[1
]}

Itoh, N ^{[1
]}

机构：

[1] Osaka Univ, Grad Sch Pharmaceut Sci, Suita, Osaka 5650871, Japan

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2005年 / 127卷 / 01期

关键词：

D O I：

10.1021/ja047018k

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Fluorometric detection of O2-• is performed based on desulfonylation of 3 to the corresponding fluoresceins 4 through nucleophilic substitution, and this fluorescing process is quite specific toward O2-• over H2O2, t-BuOOH, NaOCl, 1O2, HO•, NO•, and ONOO-. Furthermore, effects of glutathione, cytochrome P450 reductase/NADPH, and diaphorase/NADH are relatively small on the fluorescing process of probe 3 with X = Y = F, which is useful to detect O2-• released from neutrophils stimulated by phorbol myristate acetate with satisfactory sensitivity. Copyright © 2005 American Chemical Society.

引用

页码：68 / 69

页数：2

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