Efficacy of hypochlorous acid scavengers in the prevention of protein carbonyl formation

被引:94
作者
Yan, LJ
Traber, MG
Kobuchi, H
Matsugo, S
Tritschler, HJ
Packer, L
机构
[1] UNIV CALIF BERKELEY,DEPT MOLEC & CELL BIOL,BERKELEY,CA 94720
[2] ASTA MED,FRANKFURT,GERMANY
关键词
hypochlorous acid; bovine serum albumin (BSA); protein carbonyls; scavengers;
D O I
10.1006/abbi.1996.0130
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We observed that protein (bovine serum albumin) carbonyl content increases upon hypochlorite oxidation, and this increase is inhibited in a concentration dependent manner in the presence of hypochlorite scavengers. Based on this observation, we tested whether some known hypochlorite scavengers (lipoic acid, cysteine, and glutathione) and some other antioxidants (uric acid, ascorbic acid, alpha-tocopherol, and probucol) could prevent protein carbonyl formation. N-acetylcysteine, dihydrolipoic acid, cysteine, and glutathione (reduced form, GSH), which otherwise could not be tested in a previously reported 5-thio-2-nitro-benzoic acid test system, were successfully evaluated in our assay. The hypochlorite scavenging capacity of different compounds, compared by determining the IC50 (concentration which produces 50% inhibition), showed that the compounds tested have the following potency: dihydrolipoic acid > GSH, N-acetylcysteine > cysteine > S-methyl glutathione > lipoic acid, ascorbic acid > cystine, GSSG, and uric acid. No scavenging ability was observed for either alpha-tocopherol or probucol. (C) 1996 Academic Press, Inc.
引用
收藏
页码:330 / 334
页数:5
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