A PCR survey of psychrotrophic Clostridium botulinum-like isolates for the presence of BoNT genes

被引：14

作者：

Broda, DM ^{[1
]}

Boerema, JA ^{[1
]}

Bell, RG ^{[1
]}

机构：

[1] MIRINZ Food Technol & Res, Hamilton, New Zealand

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1998年 / 27卷 / 04期

关键词：

D O I：

10.1046/j.1472-765X.1998.00420.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Isolates (259) of psychrotrophic Clostridium spp. associated with either blown pack spoilage (five isolates) or slaughter stock (254 isolates) were screened for the presence of botulinum neurotoxin (BoNT) genes using degenerate PCR primers capable of amplifying A, B, E, F and G BoNT genes. No BoNT gene amplification products were detected using DNA templates from the 259 psychrotrophic isolates, including 249 isolates that showed the same 16S rRNA gene Restriction Fragment Length Polymorphism (RFLP) patterns as authentic Cl, botulinum type B. It is concluded that although the growth of such microorganisms in vacuum-packed chilled meat leads to product spoilage, it does not prejudice product safety.

引用

页码：219 / 223

页数：5

共 18 条

[1]

[Anonymous], 1974, Bergey's manual of determination bacteriology

[2] Psychrotrophic Clostridium spp associated with 'blown pack' spoilage of chilled vacuum-packed red meats and dog rolls in gas-impermeable plastic casings [J].