Emergence of enterovirus 71 "double-recombinant" strains belonging to a novel genotype D originating from southern China: first evidence for combination of intratypic and intertypic recombination events in EV71

被引：88

作者：

Yip, Cyril C. Y. ^{[4
]}

Lau, Susanna K. P. ^{[1
,2
,3
,4
]}

Zhou, Boping ^{[5
]}

Zhang, Ming-Xia ^{[5
]}

Tsoi, Hoi-Wah ^{[4
]}

Chan, Kwok-Hung ^{[4
]}

Chen, Xin-Chun ^{[5
]}

Woo, Patrick C. Y. ^{[1
,2
,3
,4
]}

Yuen, Kwok-Yung ^{[1
,2
,3
,4
]}

机构：

[1] Univ Hong Kong, Queen Mary Hosp, State Key Lab Emerging Infect Dis, Hong Kong, Hong Kong, Peoples R China

[2] Univ Hong Kong, Res Ctr Infect & Immunol, Hong Kong, Hong Kong, Peoples R China

[3] Univ Hong Kong, Carol Yu Ctr Infect, Hong Kong, Hong Kong, Peoples R China

[4] Univ Hong Kong, Dept Microbiol, Hong Kong, Hong Kong, Peoples R China

[5] Shenzhen E Lake Hosp, Shenzhen, Peoples R China

来源：

ARCHIVES OF VIROLOGY | 2010年 / 155卷 / 09期

关键词：

MOLECULAR EPIDEMIOLOGY; RNA RECOMBINATION; CORONAVIRUS HKU1; HONG-KONG; EVOLUTION; TAIWAN; OUTBREAK; SEQUENCE; HAND; VP1;

D O I：

10.1007/s00705-010-0722-0

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Hand-foot-mouth disease due to enterovirus 71 (EV71) and coxsackievirus A16 (CA16) has recently caused large outbreaks in mainland China in 2008. We performed complete genome sequencing on two EV71 (SZ/HK08-5 and SZ/HK08-6) and two CA16 (SZ/HK08-3 and SZ/HK08-7) strains from patients in Shenzhen, China. Phylogenetic, similarity plot and bootscan analyses revealed recombination between EV71 genotypes B and C at the 2A-2B junction, and between EV71 genotype B and CA16 strain G-10 in the 3C region for EV71 strains. A similar phenomenon was also found upon further gene sequencing with other EV71 strains. Recombination between CA16 strain G-10 and EV71 genotype A at the 2A-2B junction was also observed for CA16 strains. The present "double-recombinant" EV71 strains circulating in China and other EV71 subgenotype "C4" strains represent an additional genotype, D. CA16 strains should also be classified into two genotypes. This represents the first evidence for a combination of intratypic and intertypic recombination in EV71 strains.

引用

页码：1413 / 1424

页数：12

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