LspA-independent action of globomycin on Mycobacterium tuberculosis

被引：12

作者：

Banaiee, Niaz

Jacobs, William R.

Ernst, Joel D.

机构：

[1] Stanford Univ, Sch Med, Dept Pathol, Palo Alto, CA 94304 USA

[2] NYU, Sch Med, Dept Med, Div Infect Dis, New York, NY 10016 USA

[3] Albert Einstein Coll Med, Howard Hughes Med Inst, Bronx, NY 10461 USA

[4] Albert Einstein Coll Med, Dept Microbiol & Immunol, Bronx, NY 10461 USA

[5] NYU, Sch Med, Dept Microbiol, New York, NY 10016 USA

来源：

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY | 2007年 / 60卷 / 02期

关键词：

lipoproteins; lipoprotein signal peptidase; lipoprotein processing; antimycobacterials;

D O I：

10.1093/jac/dkm223

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Objectives: The objective of this study was to investigate the antimicrobial activity and specificity of globomycin, an inhibitor of lipoprotein signal peptidase II (LspA), against Mycobacterium tuberculosis. Methods: The mycobactericidal and mycobacterlostatic activity of globomycin was determined by optical density and cfu plating. The specificity of globomycin was determined by western immunoblotting using anti-MPT83 antibody. Results: Globomycin is mycobactericidal at concentrations >= 40 mg/L. However, at 80 mg/L, the processing of the lipoprotein MPT-83 is unaffected and growth-inhibitory effect of globomycin is unchanged in an IspA null mutant (Delta IspA::IspA(mut)) lacking the putative drug target. Conclusions: Globomycin kills M. tuberculosis through a mechanism that is independent of LspA.

引用

页码：414 / 416

页数：3

共 10 条

[1] Potent inhibition of macrophage responses to IFN-γ by live virulent Mycobacterium tuberculosis is independent of mature mycobacterial lipoproteins but dependent on TLR2 [J].