Light-controlled gene silencing in zebrafish embryos

被引：174

作者：

Shestopalov, Ilya A. ^{[1
]}

Sinha, Surajit ^{[1
]}

Chen, James K. ^{[1
]}

机构：

[1] Stanford Univ, Sch Med, Dept Chem & Syst Biol, Stanford, CA 94305 USA

来源：

NATURE CHEMICAL BIOLOGY | 2007年 / 3卷 / 10期

关键词：

D O I：

10.1038/nchembio.2007.30

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Functional genomic studies in zebrafish frequently use synthetic oligonucleotides called morpholinos that block RNA splicing or translation. However, the constitutive activity of these reagents limits their experimental utility. We report here the synthesis of a photoactivatable morpholino targeting the no tail ( ntl) gene. This caged reagent permits spatiotemporal gene regulation in vivo and the photochemical generation of functionally mosaic organisms.

引用

页码：650 / 651

页数：2

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