Time Evolution of the Nanoparticle Protein Corona

被引：996

作者：

Casals, Eudald ^{[1
]}

Pfaller, Tobias ^{[2
]}

Duschl, Albert ^{[2
]}

Oostingh, Gertie Janneke ^{[2
]}

Puntes, Victor ^{[1
,3
]}

机构：

[1] Inst Catala Nanotecnol, Barcelona, Spain

[2] Salzburg Univ, Dept Mol Biol, A-5020 Salzburg, Austria

[3] ICREA, Barcelona, Spain

来源：

ACS NANO | 2010年 / 4卷 / 07期

关键词：

protein corona; vroman effect; zeta potential; hydrodynamic diameter; gold nanoparticles; albuminization; GOLD NANOPARTICLES; DRUG-DELIVERY; PLASMA; ADSORPTION; BINDING; SIZE; FLUORESCENCE; PEPTIDES; SURFACES;

D O I：

10.1021/nn901372t

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

In this work, we explore the formation of the protein corona after exposure of metallic Au nanoparticles (NPs), with sizes ranging from 4 to 40 nm, to cell culture media containing 10% of fetal bovine serum. Under in vitro cell culture conditions, zeta potential measurements, UV-vis spectroscopy, dynamic light scattering and transmission electron microscope analysis were used to monitor the time evolution of the inorganic NP protein corona formation and to characterize the stability of the NPs and their surface state at every stage of the experiment. As expected, the red-shift of the surface plasmon resonance peak, as well as the drop of surface charge and the increase of the hydrodynamic diameter indicated the conjugation of proteins to NPs. Remarkably, an evolution from a loosely attached toward an irreversible attached protein corona over time was observed. Mass spectrometry of the digested protein corona revealed albumin as the most abundant component which suggests an improved biocompatibility.

引用

页码：3623 / 3632

页数：10

共 44 条

[1] Less is more in medicine - Sophisticated forms of nanotechnology will find some of their first real-world applications in biomedical research, disease diagnosis and, possibly, therapy [J].