Comparison of proteolytic enzyme activities in adults of insecticide resistant and susceptible strains of the housefly M-domestica L

被引：34

作者：

Ahmed, S

Wilkins, RM ^{[1
]}

Mantle, D

机构：

[1] Univ Newcastle Upon Tyne, Dept Agr & Environm Sci, Newcastle Upon Tyne NE1 7RU, Tyne & Wear, England

[2] Univ Newcastle Upon Tyne, Dept Neurochem, Newcastle Upon Tyne NE1 7RU, Tyne & Wear, England

来源：

INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY | 1998年 / 28卷 / 09期

关键词：

DDT; M-domestica; proteases; induction; intracellular protein catabolism; insecticide resistance; housefly;

D O I：

10.1016/S0965-1748(98)00061-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Intracellular proteolytic activity in a DDT-resistant and a susceptible strain of M. domestica was determined by assaying a comprehensive range of cytoplasmic and lysosomal enzymes. The resistant I;train showed significantly higher protease activities in whole body, head, thorax, abdomen and gut homogenates compared to the susceptible strain. The activity of alanyl- and arginyl aminopeptidase from both strains increased substantially after topical treatment with DDT at 1, 2 and 3 h, suggesting an involvement of proteolytic enzymes in the induction of detoxifying enzymes. thus indicating a possible role of the intracellular proteolytic activities in the resistance mechanism. (C) 1998 Elsevier Science Ltd. All rights reserved.

引用

页码：629 / 639

页数：11

共 42 条

[1] BLOOD DIGESTION IN THE MOSQUITO, ANOPHELES-STEPHENSI LISTON (DIPTERA, CULICIDAE), ACTIVITY AND DISTRIBUTION OF TRYPSIN, AMINOPEPTIDASE, AND ALPHA-GLUCOSIDASE IN THE MIDGUT [J].