Detection of Mycoplasma bovis in preservative-treated field milk samples

被引:68
作者
Pinnow, CC
Butler, JA
Sachse, K
Hotzel, H
Timms, LL
Rosenbusch, RF [1 ]
机构
[1] Iowa State Univ Sci & Technol, Vet Med Res Inst, Ames, IA 50011 USA
[2] Abbott Labs, Hosp Prod Div, Abbott Pk, IL 60064 USA
[3] Iowa State Univ Sci & Technol, Dept Anim Sci, Ames, IA 50011 USA
[4] Fed Inst Hlth Protect Consumers & Vet Med, Jena, Germany
关键词
Mycoplasma bovis; mycoplasma detection; polymerase chain reaction; preservative-treated milk;
D O I
10.3168/jds.S0022-0302(01)74599-7
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
Control of mycoplasmal mastitis requires individual cow milk sampling for culture and identification of Mycoplasma bovis. This sampling is time-consuming and expensive. Currently, some herds sample cows monthly with the dairy herd improvement (DHI) program, but a preservative is added to this milk that kills M. bovis. In this paper, a nested polymerase chain reaction (PCR) procedure that allows for rapid testing of preservative-treated milk is validated. The specificity of the nested PCR assay was confirmed by testing isolated nucleic acids of other organisms phylogenetically related to M. bovis or common to milk. A comparison against blind-passage culture on 53 field milk samples determined its sensitivity. Exposure of seeded milk samples to the procedure resulted in a sensitivity of 5.1 cfu equivalents per milliliter. Analysis of these results proved that the nested PCR assay was as sensitive as traditional culture and can be used on preservative-treated milk.
引用
收藏
页码:1640 / 1645
页数:6
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