Iron(II) Binding to Amyloid-β, the Alzheimer's Peptide

被引：174

作者：

Bousejra-ElGarah, Fatima

Bijani, Christian

Coppel, Yannick

Faller, Peter ^{[1
]}

Hureau, Christelle

机构：

[1] CNRS, LCC, F-31077 Toulouse, France

来源：

INORGANIC CHEMISTRY | 2011年 / 50卷 / 18期

关键词：

PRECURSOR PROTEIN; COPPER-BINDING; SENILE PLAQUE; DISEASE; ZINC; COORDINATION; FERRITIN; ALUMINUM; ENVIRONMENT; A-BETA(42);

D O I：

10.1021/ic201233b

中图分类号：

O61 [无机化学];

学科分类号：

070301 [无机化学];

摘要：

Iron has been implicated in Alzheimer's disease, but until now no direct proof of Fe-II binding to the amyloid-beta peptide (A beta) has been reported. We used NMR to evidence Fe-II coordination to full-length A beta 40 and truncated A beta 16 peptides at physiological pH and to show that the Fe-II binding site is located in the first 16 amino-acid residues. Fe-II caused selective broadening of some NMR peaks that was dependent on the Fe:A beta stoichiometry and temperature. Analysis of Fe-II broadening effect in the H-1, C-13, and 2D NMR data established that Asp1, Glu3, the three His, but not Tyr10 nor Met35 are the residues mainly involved in Fe-II coordination.

引用

收藏

页码：9024 / 9030

页数：7

相关论文

共 42 条

[1]

Soluble amyloid β1-28-copper(I)/copper(II)/iron(II) complexes are potent antioxidants in cell-free systems [J].

Baruch-Suchodolsky, Rozena ;

Fischer, Billia .

BIOCHEMISTRY, 2008, 47 (30) :7796-7806

[2]

Temperature dependence of histidine ionization constants in myoglobin [J].

Bhattacharya, S ;

Lecomte, JTJ .

BIOPHYSICAL JOURNAL, 1997, 73 (06) :3241-3256

[3]

Human Aβ1-42 reduces iron-induced toxicity in rat cerebral cortex [J].

Bishop, GM ;

Robinson, SR .

JOURNAL OF NEUROSCIENCE RESEARCH, 2003, 73 (03) :316-323

[4]

Casadesus G, 2004, J ALZHEIMERS DIS, V6, P165

[5]

Solution structure of YggX: A prokaryotic protein involved in Fe(II) trafficking [J].

Cui, Q ;

Thorgersen, MP ;

Westler, WM ;

Markley, JL ;

Downs, DM .

PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS, 2006, 62 (03) :578-586

[6]

High-resolution NMR studies of the zinc-binding site of the Alzheimer's amyloid β-peptide [J].

Danielsson, Jens ;

Pierattelli, Roberta ;

Banci, Lucia ;

Graslund, Astrid .

FEBS JOURNAL, 2007, 274 (01) :46-59

[7]

DISSOCIATION-CONSTANTS OF BRONSTED ACIDS IN D2O AND H2O - STUDIES ON POLYAZA AND POLYOXA-POLYAZA MACROCYCLES AND A GENERAL CORRELATION [J].

DELGADO, R ;

DASILVA, JJRF ;

AMORIM, MTS ;

CABRAL, MF ;

CHAVES, S ;

COSTA, J .

ANALYTICA CHIMICA ACTA, 1991, 245 (02) :271-282

[8]

Pulse EPR Spectroscopy Reveals the Coordination Sphere of Copper(II) Ions in the 1-16 Amyloid-β Peptide: A Key Role of the First Two N-Terminus Residues [J].

Dorlet, Pierre ;

Gambarelli, Serge ;

Faller, Peter ;

Hureau, Christelle .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2009, 48 (49) :9273-9276

[9]

Alanine-2 Carbonyl is an Oxygen Ligand in Cu2+ Coordination of Alzheimer's Disease Amyloid-β Peptide - Relevance to N-Terminally Truncated Forms [J].

Drew, Simon C. ;

Masters, Colin L. ;

Barnham, Kevin J. .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2009, 131 (25) :8760-+

[10]

Pleomorphic Copper Coordination by Alzheimer's Disease Amyloid-β Peptide [J].

Drew, Simon C. ;

Noble, Christopher J. ;

Masters, Colin L. ;

Hanson, Graeme R. ;

Barnham, Kevin J. .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2009, 131 (03) :1195-1207

← 1 2 3 4 5 →