Single electrode conductivity detection for electrophoretic separation systems

被引：42

作者：

Prest, JE ^{[1
]}

Baldock, SJ ^{[1
]}

Bektas, N ^{[1
]}

Fielden, PR ^{[1
]}

Brown, BJT ^{[1
]}

机构：

[1] Univ Manchester, Inst Sci & Technol, Dept Instrumentat & Analyt Sci, Manchester M60 1QD, Lancs, England

来源：

JOURNAL OF CHROMATOGRAPHY A | 1999年 / 836卷 / 01期

基金：

英国工程与自然科学研究理事会;

关键词：

conductivity detection; detection; electrophoresis; isotachophoresis; instrumentation; sodium; potassium; metal cations;

D O I：

10.1016/S0021-9673(99)00061-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Various types of detectors have been utilised in electrophoretic separation systems, but conductivity detection provides the obvious choice for detecting ionic substances. This paper describes a new design of conductivity detector utilising a single, on column, sensing electrode. This new design is seen as offering the twin advantages of much higher resolution, than has previously been possible, and simple construction. The detector has been evaluated using separations of sodium and potassium in capillary isotachophoretic and miniaturised planar polymer isotachophoretic separation systems. Good linearity has been found and the limit of detection of sodium calculated to be 0.43 mM. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：59 / 65

页数：7

共 13 条

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[2]

EVERAERTS FM, 1972, J CHROMATOGR, V73, P193

[3]

EVERAERTS FM, 1976, ISOTACHOPHORESIS

[4] ONLINE FIBER OPTIC UV DETECTION CELL AND CONDUCTIVITY CELL FOR CAPILLARY ZONE ELECTROPHORESIS [J].