Relating a calcium indicator signal to the unperturbed calcium concentration time-course

被引:12
作者
Borst, Alexander [1 ]
Abarbanel, Henry D. I. [2 ,3 ]
机构
[1] Max Planck Inst Neurobiol, Martinsried, Germany
[2] Univ Calif San Diego, Dept Phys, San Diego, CA 92103 USA
[3] Univ Calif San Diego, Marine Phys Lab, Scripps Inst Oceanog, San Diego, CA 92103 USA
来源
THEORETICAL BIOLOGY AND MEDICAL MODELLING | 2007年 / 4卷
关键词
D O I
10.1186/1742-4682-4-7
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Background: Optical indicators of cytosolic calcium levels have become important experimental tools in systems and cellular neuroscience. Indicators are known to interfere with intracellular calcium levels by acting as additional buffers, and this may strongly alter the time-course of various dynamical variables to be measured. Results: By investigating the underlying reaction kinetics, we show that in some ranges of kinetic parameters one can explicitly link the time dependent indicator signal to the time-course of the calcium influx, and thus, to the unperturbed calcium level had there been no indicator in the cell.
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页数:13
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