Expression analyses of sex steroid-regulated genes in neonatal rat hypothalamus

被引:14
作者
Yonehara, K [1 ]
Suzuki, M [1 ]
Yamanouchi, K [1 ]
Nishihara, M [1 ]
机构
[1] Univ Tokyo, Dept Vet Physiol, Tokyo 1138657, Japan
关键词
DNA microarray; gene expression; estrogen; androgen; sexual differentiation of brain;
D O I
10.1262/jrd.49.547
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
Estrogen plays an important role in sexual differentiation of the brain in rats during the perinatal period. To elucidate molecular mechanisms underlying sexual differentiation of the brain, in this study we investigated genes differentially expressed between sexes or induced to express by estrogen in neonatal rat hypothalamus using DNA microarray analysis in combination with real-time RT-PCR. It was found that the levels of expression of the genes encoding glutamic acid decarboxylase 65 and coronin 1b were higher in male than female hypothalamus on postnatal day (PN) 5 and those of collagen type 3 alpha1 and thioredoxin reductase 2 genes in female hypothalamus on PN5 were decreased and increased, respectively, by treatment with estradiol on PN2. Then the developmental changes in the expression of these 4 genes were examined from 1 day before the parturition to PN9, and they all showed sexual dimorphic patterns. In addition, dependence of the expression of these genes on either estradiol, testosterone or dihydrotestosterone during the neonatal period was confirmed. These results suggest that these four genes are involved in sexual differentiation of the rat brain, and that androgen per se as well as estrogen may take part in the processes.
引用
收藏
页码:547 / 552
页数:6
相关论文
共 24 条
[1]  
Arai Y, 1996, NEUROSCI RES, V25, P403
[2]   SYNAPSE FORMATION OF HYPOTHALAMIC ARCUATE NUCLEUS DURING POSTNATAL-DEVELOPMENT IN FEMALE RAT AND ITS MODIFICATION BY NEONATAL ESTROGEN-TREATMENT [J].
ARAI, Y ;
MATSUMOTO, A .
PSYCHONEUROENDOCRINOLOGY, 1978, 3 (01) :31-45
[3]  
ARNOLD AP, 1984, ANNU REV NEUROSCI, V7, P423
[4]   Collagens and atherosclerosis [J].
Barnes, MJ ;
Farndale, RW .
EXPERIMENTAL GERONTOLOGY, 1999, 34 (04) :513-525
[5]   Oestrogen as a neuroprotective hormone [J].
Behl, C .
NATURE REVIEWS NEUROSCIENCE, 2002, 3 (06) :433-442
[6]   EFFECTS OF 17-BETA-ESTRADIOL ON THE BIOSYNTHESIS OF COLLAGEN IN CULTURED BOVINE AORTIC SMOOTH-MUSCLE CELLS [J].
BELDEKAS, JC ;
SMITH, B ;
GERSTENFELD, LC ;
SONENSHEIN, GE ;
FRANZBLAU, C .
BIOCHEMISTRY, 1981, 20 (08) :2162-2167
[7]   Sex differences in glutamic acid decarboxylase mRNA in neonatal rat brain: Implications for sexual differentiation [J].
Davis, AM ;
Grattan, DR ;
Selmanoff, M ;
McCarthy, MM .
HORMONES AND BEHAVIOR, 1996, 30 (04) :538-552
[8]   The coronin family of actin-associated proteins [J].
de Hostos, EL .
TRENDS IN CELL BIOLOGY, 1999, 9 (09) :345-350
[9]   HORMONAL MODIFICATION OF THE NUMBER OF TOTAL AND LATE-ARISING NEURONS IN THE CENTRAL PART OF THE MEDIAL PREOPTIC NUCLEUS OF THE RAT [J].
DODSON, RE ;
SHRYNE, JE ;
GORSKI, RA .
JOURNAL OF COMPARATIVE NEUROLOGY, 1988, 275 (04) :623-629
[10]   E2F activity is biphasically regulated by androgens in LNCaP cells [J].
Hofman, K ;
Swinnen, JV ;
Verhoeven, G ;
Heyns, W .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2001, 283 (01) :97-101