Inductively coupled plasma mass spectrometric analysis of the total amount of platinum in DNA extracts from peripheral blood mononuclear cells and tissue from patients treated with cisplatin

被引：38

作者：

Brouwers, E. E. M. ^{[1
]}

Tibben, M. M. ^{[1
]}

Pluim, D. ^{[2
]}

Rosing, H. ^{[1
]}

Boot, H. ^{[3
]}

Cats, A. ^{[3
]}

Schellens, J. H. M. ^{[3
,4
]}

Beijnen, J. H. ^{[1
,4
]}

机构：

[1] Netherlands Canc Inst, Dept Pharm & Pharmacol, Slotervaart Hosp, NL-1066 EC Amsterdam, Netherlands

[2] Netherlands Canc Inst, Dept Expt Therapy, Antoni Van Leeuwenhoek Hosp, NL-1066 CX Amsterdam, Netherlands

[3] Netherlands Canc Inst, Dept Med Oncol, Antoni Van Leeuwenhoek Hosp, NL-1066 CX Amsterdam, Netherlands

[4] Univ Utrecht, Beta Fac, Dept Pharmaceut Sci, Div Biomed Anal,Sect Drug Toxicol, NL-3508 TB Utrecht, Netherlands

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2008年 / 391卷 / 02期

关键词：

ICP-MS; platinum; DNA adducts; peripheral blood mononuclear cells; tissue;

D O I：

10.1007/s00216-008-2034-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

We present a highly sensitive method for the determination of platinum (Pt) in DNA extracts of peripheral blood mononuclear cells (PBMCs) and tissue samples from patients treated with cisplatin. The method is based on the measurement of Pt by inductively coupled plasma mass spectrometry (ICP-MS) and allows quantification of Pt-DNA adducts in PBMCs isolated from 10 mL blood and 1 mg tissue. The lower limit of quantification is 0.75 pg Pt or 7.5 fg Pt mu g(-1) DNA when using 100 mu g DNA. The method proved to be accurate and precise. The results obtained using the ICP-MS method were in good agreement with results from the alternative (32)P-postlabelling assay. The ICP-MS method was, however, more sensitive and proved to be less laborious. The advantages of the presented ICP-MS technique were demonstrated by the analysis of PBMCs, normal gastric tissue, and gastric tumour tissue of patients treated with cisplatin.

引用

页码：577 / 585

页数：9

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