Simultaneous determination of propanol and butanol by a fluorometric enzymatic method based on the intrinsic fluorescence of the enzyme

被引：5

作者：

Sierra, JF

de Pedro, M

de Marcos, S

Galbán, J ^{[1
]}

Castillo, JR

Ubide, C

机构：

[1] Univ Zaragoza, Fac Sci, Dept Analyt Chem, GEAS, E-50009 Zaragoza, Spain

[2] Univ Basque Country, Fac Chem, Dept Appl Chem, E-20080 San Sebastian, Spain

来源：

FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY | 1999年 / 363卷 / 07期

关键词：

Enzyme; Alcohol; Mathematical Model; Butanol; Propanol;

D O I：

10.1007/s002160051278

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The application of the intrinsic fluorescence of alcohol dehydrogenase (ADH) for the simultaneous determination of propanol and butanol was investigated by using only one kinetic run. An absolute calibration method (based on a mathematical model derived from the enzymatic reaction) and a multivariate calibration method (partial least squares regression, PLSR) were tested for this purpose. Both methods were applied to synthetic samples of both alcohols with good results.

引用

页码：713 / 715

页数：3