Expression profile of the genes for rice cation/H+ exchanger family and functional analysis in yeast

被引：68

作者：

Kamiya, T ^{[1
]}

Akahori, T ^{[1
]}

Maeshima, M ^{[1
]}

机构：

[1] Nagoya Univ, Lab Cell Dynam, Grad Sch Bioagr Sci, Chikusa Ku, Nagoya, Aichi 4648601, Japan

来源：

PLANT AND CELL PHYSIOLOGY | 2005年 / 46卷 / 10期

基金：

日本学术振兴会;

关键词：

cation/H+ exchanger; gene expression; Oryza sativa; vacuole; yeast;

D O I：

10.1093/pcp/pci173

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

We identified five cation/H+ exchangers (CAX) from rice, and phylogenetically divided them into two clusters. Gene expression and absolute amounts of mRNA in different organs were analyzed by real-time PCR. OsCAX1a showed high expression in almost all organs. OsCAX1b and OsCAX1c were detected in a limited number of organs and their expression levels were very low. The mRNA levels of OsCAX2 and OsCAX3 varied with the organ. OsCAXs were heterologously expressed in yeast to characterize the ion transport activity. All exchangers, except for OsCAX2, conferred tolerance to calcium. OsCAX1a and OsCAX3 conferred tolerance to manganese. The diversity of expression sites and substrates suggest the broad range function of CAX.

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收藏

页码：1735 / 1740

页数：6

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