The complete DNA sequence of the mitochondrial genome of Physarum polycephalum

被引：48

作者：

Takano, H ^{[1
]}

Abe, T

Sakurai, R

Moriyama, Y

Miyazawa, Y

Nozaki, H

Kawano, S

Sasaki, N

Kuroiwa, T

机构：

[1] Kumamoto Univ, Fac Sci, Dept Biol Sci, Kumamoto 8608555, Japan

[2] Univ Tokyo, Grad Sch Sci, Dept Biol Sci, Tokyo 113, Japan

[3] Univ Tokyo, Grad Sch Frontier Sci, Dept Integrated Biosci, Tokyo 113, Japan

来源：

MOLECULAR AND GENERAL GENETICS | 2001年 / 264卷 / 05期

关键词：

mitochondria; mtDNA; Physarum polycephalum; RNA editing;

D O I：

10.1007/s004380000357

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The complete sequence of the mitochondrial DNA (mtDNA) of the true slime mold Physarum polycephalum has been determined. The mtDNA is a circular 62,862-bp molecule with an A+T contest of 74.1%. A search with the program BLAST X identified the protein-coding regions. The mitochondrial genome of P. polycephalum was predicted to contain genes coding for 12 known proteins [for three cytochrome ( oxidase subunits, apocytochrome b, two F1Fo-ATPase subunits, five NADH dehydrogenase (nad) subunits, and one ribosomal protein], two rRNA genes, and five tRNA genes. However, the predicted ORFs are not all in the same frame, because mitochondrial RNA in P. polycephalum undergoes RNA editing to produce functional RNAs. The nucleotide sequence of an nad7 cDNA showed that 51 nucleotides were inserted at 46 sites in the mRNA. No guide RNA-like sequences were observed in the mtDNA of P. polycephalum. Comparison with reported Physarum mtDNA sequences suggested that sites of RNA editing vary among strains. In the Physarum mtDNA, 20 ORFs of over 300 nucleotides were found and ORFs 14-19 are transcribed.

引用

页码：539 / 545

页数：7

共 33 条

[1] In vitro DNA fragmentation of mitochondrial DNA caused by single-stranded breakage related to macroplasmodial senescence of the true slime mold, Physarum polycephalum [J].

Abe, T ;

Takano, H ;

Sasaki, N ;

Mori, K ;

Kawano, S .

CURRENT GENETICS, 2000, 37 (02) :125-135

[2]

ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1006/jmbi.1990.9999

[3] Insertional editing of mitochondrial tRNAs of Physarum polycephalum and Didymium nigripes [J].

Antes, T ;

Costandy, H ;

Mahendran, R ;

Spottswood, M ;

Miller, D .

MOLECULAR AND CELLULAR BIOLOGY, 1998, 18 (12) :7521-7527

[4] A MODEL FOR RNA EDITING IN KINETOPLASTID MITOCHONDRIA - GUIDE RNA MOLECULES TRANSCRIBED FROM MAXICIRCLE DNA PROVIDE THE EDITED INFORMATION [J].

BLUM, B ;

BAKALARA, N ;

SIMPSON, L .

CELL, 1990, 60 (02) :189-198

[5] TRYPANOSOMA-BRUCEI MINICIRCLES ENCODE MULTIPLE GUIDE RNAS WHICH CAN DIRECT EDITING OF EXTENSIVELY OVERLAPPING SEQUENCES [J].

CORELL, RA ;

FEAGIN, JE ;

RILEY, GR ;

STRICKLAND, T ;

GUDERIAN, JA ;

MYLER, PJ ;

STUART, K .

NUCLEIC ACIDS RESEARCH, 1993, 21 (18) :4313-4320

[6]

COSTANDY H, 1999, RNA EDITING COX3 MRN

[7]

Dee J., 1982, CELL BIOL PHYSARUM D, P211

[8] IDENTIFICATION OF PROTEIN CODING REGIONS BY DATABASE SIMILARITY SEARCH [J].

GISH, W ;

STATES, DJ .

NATURE GENETICS, 1993, 3 (03) :266-272

[9]

GOTT JM, 1993, J BIOL CHEM, V268, P25483

[10] Genome structure and gene content in protist mitochondrial DNAs [J].

Gray, MW ;

Lang, BF ;

Cedergren, R ;

Golding, GB ;

Lemieux, C ;

Sankoff, D ;

Turmel, M ;

Brossard, N ;

Delage, E ;

Littlejohn, TG ;

Plante, I ;

Rioux, P ;

Saint-Louis, D ;

Zhu, Y ;

Burger, G .

NUCLEIC ACIDS RESEARCH, 1998, 26 (04) :865-878

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