cdc42 regulates the exit of apical and basolateral proteins from the trans-Golgi network

被引:149
作者
Müsch, A [1 ]
Cohen, D
Kreitzer, G
Rodriguez-Boulan, E
机构
[1] Cornell Univ, Joan & Sanford Weill Med Coll, Dyson Inst Vis Res, Dept Biochem, New York, NY 10021 USA
[2] Cornell Univ, Joan & Sanford Weill Med Coll, Dyson Inst Vis Res, Dept Cell Biol, New York, NY 10021 USA
关键词
cdc42; Golgi; protein transport;
D O I
10.1093/emboj/20.9.2171
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
It is well established that Rho-GTPases regulate vesicle fusion and fission events at the plasma membrane through their modulatory role on the cortical actin cytoskeleton. In contrast, their effects on intracellular transport processes and actin pools are less clear. It was recently shown that cdc42 associates with the Golgi apparatus in an ARF-dependent manner, similarly to coat proteins involved in vesicle formation and to several actin-binding proteins. We report here that mutants of cdc42 inhibited the exit of basolateral proteins from the trans-Golgi network (TGN), while stimulating the exit of an apical marker, in two different transport assays. This regulation may result from modulation of the actin cytoskeleton, as GTPase-deficient cdc42 depleted a perinuclear actin pool that rapidly exchanges with exogenous fluorescent actin.
引用
收藏
页码:2171 / 2179
页数:9
相关论文
共 45 条
[1]  
Babià T, 1999, J CELL SCI, V112, P477
[2]   GOLGI SPECTRIN - IDENTIFICATION OF AN ERYTHROID BETA-SPECTRIN HOMOLOG ASSOCIATED WITH THE GOLGI-COMPLEX [J].
BECK, KA ;
BUCHANAN, JA ;
MALHOTRA, V ;
NELSON, WJ .
JOURNAL OF CELL BIOLOGY, 1994, 127 (03) :707-723
[3]  
Brown VA, 1998, STUD FRANCESI, V42, P9
[4]   Identification of two distinct mechanisms of phagocytosis controlled by different Rho GTPases [J].
Caron, E ;
Hall, A .
SCIENCE, 1998, 282 (5394) :1717-1721
[5]   The role of ankyrin and spectrin in membrane transport and domain formation [J].
De Matteis, MA ;
Morrow, JS .
CURRENT OPINION IN CELL BIOLOGY, 1998, 10 (04) :542-549
[6]  
di Campli A, 1999, CELL MOTIL CYTOSKEL, V43, P334, DOI 10.1002/(SICI)1097-0169(1999)43:4<334::AID-CM6>3.0.CO
[7]  
2-3
[8]   Sorting and signaling at the Golgi complex [J].
Donaldson, JG ;
Lippincott-Schwartz, J .
CELL, 2000, 101 (07) :693-696
[9]   CDC42 AND RAC1 CONTROL DIFFERENT ACTIN-DEPENDENT PROCESSES IN THE DROSOPHILA WING DISC EPITHELIUM [J].
EATON, S ;
AUVINEN, P ;
LUO, LQ ;
JAN, YN ;
SIMONS, K .
JOURNAL OF CELL BIOLOGY, 1995, 131 (01) :151-164
[10]   CELLULAR EXPRESSION OF LIVER AND NEURAL CELL-ADHESION MOLECULES AFTER TRANSFECTION WITH THEIR CDNAS RESULTS IN SPECIFIC CELL CELL BINDING [J].
EDELMAN, GM ;
MURRAY, BA ;
MEGE, RM ;
CUNNINGHAM, BA ;
GALLIN, WJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (23) :8502-8506