Caspases: Preparation and characterization

被引：160

作者：

Stennicke, HR ^{[1
]}

Salvesen, GS ^{[1
]}

机构：

[1] Burnham Inst, Program Apoptosis & Cell Death Res, La Jolla, CA 92037 USA

来源：

METHODS-A COMPANION TO METHODS IN ENZYMOLOGY | 1999年 / 17卷 / 04期

关键词：

D O I：

10.1006/meth.1999.0745

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Caspases and their involvement in programmed cell death have been an area of significant interest since their initial identification in 1992. To facilitate the search for new components involved in cell death, and to aid researchers in understanding the interactions between currently known cell death proteins, we describe a number of techniques commonly used in the preparation and characterization of caspases. (C) 1999 Academic Press.

引用

页码：313 / 319

页数：7

共 29 条

[1] Involvement of MACH, a novel MORT1/FADD-interacting protease, in Fas/APO-1- and TNF receptor-induced cell death [J].

Boldin, MP ;

Goncharov, TM ;

Goltsev, YV ;

Wallach, D .

CELL, 1996, 85 (06) :803-815

[2] Apopain/CPP32 cleaves proteins that are essential for cellular repair: A fundamental principle of apoptotic death [J].

CasciolaRosen, L ;

Nicholson, DW ;

Chong, T ;

Rowan, KR ;

Thornberry, NA ;

Miller, DK ;

Rosen, A .

JOURNAL OF EXPERIMENTAL MEDICINE, 1996, 183 (05) :1957-1964

[3] Caspase cleavage of keratin 18 and reorganization of intermediate filaments during epithelial cell apoptosis [J].

Caulin, C ;

Salvesen, GS ;

Oshima, RG .

JOURNAL OF CELL BIOLOGY, 1997, 138 (06) :1379-1394

[4] MOLECULAR-CLONING OF THE INTERLEUKIN-1-BETA CONVERTING ENZYME [J].

CERRETTI, DP ;

KOZLOSKY, CJ ;

MOSLEY, B ;

NELSON, N ;

VANNESS, K ;

GREENSTREET, TA ;

MARCH, CJ ;

KRONHEIM, SR ;

DRUCK, T ;

CANNIZZARO, LA ;

HUEBNER, K ;

BLACK, RA .

SCIENCE, 1992, 256 (5053) :97-100

[5]

Chinnaiyan AM, 1996, CURR BIOL, V6, P555

[6] Caspases: the executioners of apoptosis [J].

Cohen, GM .

BIOCHEMICAL JOURNAL, 1997, 326 :1-16

[7] Proteolysis of poly(ADP-ribose) polymerase by caspase 3: Kinetics of cleavage of mono(ADP-ribosyl)ated and DNA-bound substrates [J].

D'Amours, D ;

Germain, M ;

Orth, K ;

Dixit, VM ;

Poirier, GG .

RADIATION RESEARCH, 1998, 150 (01) :3-10

[8] ICE-LAP6, a novel member of the ICE/Ced-3 gene family, is activated by the cytotoxic T cell protease granzyme B [J].

Duan, HJ ;

Orth, K ;

Chinnaiyan, AM ;

Poirier, GG ;

Froelich, CJ ;

He, WW ;

Dixit, VM .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (28) :16720-16724

[9] In vitro activation of CPP32 and Mch3 by Mch4, a novel human apoptotic cysteine protease containing two FADD-like domains [J].

FernandesAlnemri, T ;

Armstrong, RC ;

Krebs, J ;

Srinivasula, SM ;

Wang, L ;

Bullrich, F ;

Fritz, LC ;

Trapani, JA ;

Tomaselli, KJ ;

Litwack, G ;

Alnemri, ES .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (15) :7464-7469

[10] The CARD domain: A new apoptotic signalling motif [J].

Hofmann, K ;

Bucher, P .

TRENDS IN BIOCHEMICAL SCIENCES, 1997, 22 (05) :155-156

← 1 2 3 →