Gas-phase photocatalytic degradation and detoxification of o-toluidine: Degradation mechanism and Salmonella mutagenicity assessment of mixed gaseous intermediates

The photocatalytic degradation of toluidine over titanium oxide (TiO(2)) thin films under UV irradiation was investigated. The degradation efficiency of 98.7% was obtained for a toluidine concentration of about 4500 mu g L(-1) and illumination of 240 min. The degradation intermediates produced during photocatalytic oxidation were identified using Fourier transform-infrared spectrometry (FTIR) and gas chromatography-mass spectrometry (GC-MS). Only a small amount of intermediates, including phenol and toluene, were found in the gas phase. Many other trace amount intermediates, such as 2-hydroxybenzaldehyde, 2-nitrobenzaldehyde, 2-hydroxybenzenemethanol, 2-hydroxybenzoic acid, phenol etc., were detected on the TiO(2) surface. An Ames assay of the Salmonella typhimurium strains TA98 and TA100 was employed to evaluate the mutagenicity of toluidine and its gaseous photocatalytic degradation intermediates. With or without rat liver microsomal fraction (S9 mix) activation, neither toluidine nor its gaseous intermediates presented mutagenic activity against strains TA98 (+/- S9) and TA100 (-S9) at all tested doses. Toluidine, however, can induce a weak positive response to the TA100 strain with an S9 mix at doses as high as 4000 mu g plate(-1). An increase of revertants per plate was obtained after 30 min photocatalysis in the TA100 strain with S9 mix. As reaction time further increased, photocatalytic technology exhibited the ability to completely and efficiently detoxify toluidine. Both our chemical analysis and toxic evaluation indicate that all mutagenic intermediates in the gas can be completely eliminated within 240 min, which further suggests that photocatalytic technology is an effective approach for degrading aromatic amines. (C) 2010 Elsevier B.V. All rights reserved.