Parallel human genome analysis: Microarray-based expression monitoring of 1000 genes

被引：1232

作者：

Schena, M

Shalon, D

Heller, R

Chai, A

Brown, PO

Davis, RW

机构：

[1] SYNTENI,PALO ALTO,CA 94306

[2] STANFORD UNIV,MED CTR,DEPT BIOCHEM,STANFORD,CA 94305

[3] STANFORD UNIV,MED CTR,BECKMAN CTR,HOWARD HUGHES MED INST,STANFORD,CA 94305

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1996年 / 93卷 / 20期

关键词：

human genome project; DNA chip; gene discovery; T cell;

D O I：

10.1073/pnas.93.20.10614

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Microarrays containing 1046 human cDNAs of unknown sequence were printed on glass with high-speed robotics. These 1.0-cm(2) DNA ''chips'' were used to quantitatively monitor differential expression of the cognate human genes using a highly sensitive two-color hybridization assay, Array elements that displayed differential expression patterns under given experimental conditions were characterized by sequencing, The identification of known and novel heat shock and phorbol ester-regulated genes in human T cells demonstrates the sensitivity of the assay. Parallel gene analysis with microarrays provides a rapid and efficient method for large-scale human gene discovery.

引用

页码：10614 / 10619

页数：6

共 33 条

[1] Serial analysis of gene expression: ESTs get smaller [J].