Enzyme Immunassay of Alternariol for the Assessment of Risk of Agricultural Products Contamination

被引:23
作者
Burkin, A. A. [1 ]
Kononenko, G. P. [1 ]
机构
[1] Russian Acad Agr Sci, All Russia Res Inst Vet Sanitat Hyg & Ecol, Moscow 123022, Russia
关键词
TOXINS; MYCOTOXINS; IMMUNOASSAYS;
D O I
10.1134/S0003683811010030
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A highly specific test system based on polyclonal rabbit antibodies targeting a conjugate of alternariol and bovine serum albumin prepared by formaldehyde condensation has been developed for the first time and shown to have the sensitivity of 0.4 ng/ml in the indirect competitive determination of alternariol. The possibility of applying the assay for assessment of the intensity of alternariol contamination of corn, animal feed, and raw materials used for the preparation of the latter has been demonstrated.
引用
收藏
页码:72 / 76
页数:5
相关论文
共 16 条
[1]  
Belyakova G. A., 1992, Mikologiya i Fitopatologiya, V26, P183
[2]  
BELYAKOVA GA, 1990, MIKOL FITOPATOL, V24, P128
[3]  
BETINA V, 1993, CHROMATOGRAPHY MYCOT
[4]  
Cole R.J., 1981, HDB TOXIC FUNGAL MET
[5]  
Grabarkiewicz-Szczesna J, 1989, Mycotoxin Res, V5, P77, DOI 10.1007/BF03192125
[6]  
GRUBERSCHLEY S, 1988, LANDWIRT FORSCH, V41, P11
[7]  
HERMANSON GT, 1996, BIOCONJUGATE TECHNIQ
[8]  
KISLYAKOVA OS, 2001, PROBL VET SANIT EKOL, V111, P115
[9]   Alternaria mycotoxins in weathered wheat from China [J].
Li, FQ ;
Yoshizawa, T .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2000, 48 (07) :2920-2924
[10]  
Logrieco A., 1988, Microbiologie - Aliments - Nutrition, V6, P13