Profiling hydroxycinnamoyl-coenzyme A thioesters: Unlocking the back door of phenylpropanoid metabolism

被引：9

作者：

Qualley, Anthony V. ^{[1
]}

Cooper, Bruce R. ^{[2
]}

Dudareva, Natalia ^{[1
]}

机构：

[1] Purdue Univ, Dept Hort & Landscape Architecture, W Lafayette, IN 47907 USA

[2] Purdue Univ, Bindley Biosci Ctr, Metabolite Profiling Facil, W Lafayette, IN 47907 USA

来源：

ANALYTICAL BIOCHEMISTRY | 2012年 / 420卷 / 02期

基金：

美国国家科学基金会;

关键词：

Hydroxycinnamoyl-CoA esters; Plants; Acyl-CoA esters; Metabolite profiling; MS/MS; PLANT-TISSUES; ACID; QUANTIFICATION; BIOSYNTHESIS; PATHWAYS; ESTERS; COA;

D O I：

10.1016/j.ab.2011.09.010

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In plants, 20 to 30% of photosynthetically fixed carbon is directed toward lignin and other phenylpropanoid compounds for which hydroxycinnamoyl-coenzyme A (CoA) esters are key intermediates. CoA thioesters, ubiquitous metabolites found in all living cells (often at trace levels), have traditionally been challenging to measure. Here we report a hydrophilic interaction liquid chromatography (HILIC) method, coupled with tandem mass spectrometry (MS/MS), that allows simultaneous sensitive quantification of previously undetectable hydroxycinnamoyl-CoA esters and an extended range of acyl-CoAs from plant tissues. This method provides rapid liquid chromatography (LC) analysis (10 min/sample) and the ability for qualitative assessment of acyl-CoAs by MS/MS precursor ion scanning. (C) 2011 Elsevier Inc. All rights reserved.

引用

页码：182 / 184

页数：3

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