Novel proteoglycan linkage tetrasaccharides of human urinary soluble thrombomodulin, SO4-3GlcAβ1-3Galβ1-3(±Siaα2-6)Galβ1-4Xyl

被引：39

作者：

Wakabayashi, H

Natsuka, S

Mega, T

Otsuki, N

Isaji, M

Naotsuka, M

Koyama, S

Kanamori, T

Sakai, K

Hase, S ^{[1
]}

机构：

[1] Osaka Univ, Grad Sch Sci, Dept Chem, Toyonaka, Osaka 5600043, Japan

[2] Mochida Pharmaceut Co Ltd, Pharmaceut Lab, Fujieda, Shizuoka 4268640, Japan

[3] Mochida Pharmaceut Co Ltd, Biosci Res Lab, Kita Ku, Tokyo 1158515, Japan

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 1999年 / 274卷 / 09期

关键词：

D O I：

10.1074/jbc.274.9.5436

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

O-linked sugar chains with xylose as a reducing end linked to human urinary soluble thrombomodulin were studied. Sugar chains were Liberated by hydrazinolysis followed by N-acetylation and tagged with 2-aminopyridine. Two fractions containing pyridylaminated Xyl as a reducing end were collected. Their structures were determined by partial acid hydrolysis, two-dimensional sugar mapping combined with exoglycosidase digestions, methylation analysis, mass spectrometry, and NMR as SO4-3GlcA beta 1-3Gal beta 1-3(+/-Sia alpha 2-6)Gal beta 1-4Xyl. These sugar chains could bind to an HNK-1 monoclonal antibody. This is believed to be the first example of a proteoglycan linkage tetrasaccharide with glucuronic acid 3-sulfate and sialic acid.

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页码：5436 / 5442

页数：7

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