Synthetic macrocyclic peptidomimetics as tunable pH probes for the fluorescence imaging of acidic organelles in live cells

被引：144

作者：

Galindo, F

Burguete, MI

Vigara, L

Luis, SV

Kabir, N

Gavrilovic, J

Russell, DA

机构：

[1] Univ Jaume 1, Dept Quim Inorgan & Organ, Castellon de La Plana 12071, Spain

[2] Univ E Anglia, Sch Chem Sci & Pharm, Norwich NR4 7TJ, Norfolk, England

[3] Univ E Anglia, Sch Biol Sci, Norwich NR4 7TJ, Norfolk, England

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2005年 / 44卷 / 40期

基金：

英国惠康基金;

关键词：

biosensors; fluorescent probes; macrocycles; peptidomimetics; protonation;

D O I：

10.1002/anie.200501920

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Chemical Equation Presented) Acid test: Macrocyclic compounds that comprise a 9,10-anthracene subunit linked by a C2-symmetric peptidomimetic chain (see formula) are useful as fluorescent probes in a pH range of biomedical interest. Changes in the size of the macrocycle (n = 3,4,6,8) produce a shift in the pKa values. Experiments with live mouse macrophage cells show the selective localization of the probe in the acidic organelles (see differential interference contrast image). © 2005 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：6504 / 6508

页数：5

共 37 条

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