Inhibition of airway remodeling in IL-5-deficient mice

被引：319

作者：

Cho, JY ^{[1
]}

Miller, M ^{[1
]}

Baek, KJ ^{[1
]}

Han, JW ^{[1
]}

Nayar, J ^{[1
]}

Lee, SY ^{[1
]}

McElwain, K ^{[1
]}

McElwain, S ^{[1
]}

Friedman, S ^{[1
]}

Broide, DH ^{[1
]}

机构：

[1] Univ Calif San Diego, Dept Med, La Jolla, CA 92093 USA

来源：

JOURNAL OF CLINICAL INVESTIGATION | 2004年 / 113卷 / 04期

关键词：

D O I：

10.1172/JCI200419133

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

To determine the role of IL-5 in airway remodeling, IL-5-deficient and WT mice were sensitized to OVA and challenged by repetitive administration of OVA for 3 months. IL-5-deficient mice had significantly less peribronchial fibrosis (total lung collagen content, peribronchial collagens III and V) and significantly less peribronchial smooth muscle (thickness of peribronchial smooth muscle layer, alpha-smooth muscle actin immunostaining) compared with WT mice challenged with OVA. WT mice had a significant increase in the number of peribronchial cells staining positive for major basic protein and TGF-beta. In contrast, IL-5-deficient mice had a significant reduction in the number of peribronchial cells staining positive for major basic protein, which was paralleled by a similar reduction in the number of cells staining positive for TGF-beta, suggesting that eosinophils are a significant source of TGF-beta in the remodeled airway. OVA challenge induced significantly higher levels of airway epithelial alpha(V)beta(6) integrin expression, as well as significantly higher levels of bioactive lung TGF-beta in WT compared with IL-5-deficient mice. Increased airway epithelial. expression Of alpha(V)beta(6) integrin may contribute to the increased activation of latent TGF-beta. These results suggest an important role for IL-5, eosinophils, alpha(V)beta(6), and TGF-beta in airway remodeling.

引用

页码：551 / 560

页数：10

共 34 条

[1] Mechanisms of disease:: Role of transforming growth factor β in human disease. [J].