Assessment of fiberoptic near-infrared Raman spectroscopy for diagnosis of bladder and prostate cancer

被引:172
作者
Crow, P
Molckovsky, A
Stone, N
Uff, J
Wilson, B
Wongkeesong, LM
机构
[1] Gloucestershire Royal Hosp, Biophoton Grp, Gloucester GL1 3NN, Glos, England
[2] Univ Toronto, Univ Hlth Network, Dept Med Biophys, Toronto, ON, Canada
[3] Mayo Clin, Dept Internal Med, Rochester, MN USA
关键词
D O I
10.1016/j.urology.2004.12.058
中图分类号
R5 [内科学]; R69 [泌尿科学(泌尿生殖系疾病)];
学科分类号
1002 ; 100201 ;
摘要
Objectives. To determine whether a fiberoptic Raman system, suitable for in vivo use, is able to differentiate between benign and malignant bladder and prostate pathologic findings in vitro. Raman spectroscopy is an optical technique that provides a measure of the molecular composition of tissue by analyzing the way that tissue scatters laser light. Laboratory studies have shown that the technique can be used to identify and characterize transitional cell carcinoma and prostate adenocarcinoma in vitro. Methods. A total of 220 Raman spectra were recorded from 29 snap-frozen bladder samples collected at cystoscopic procedures, and 197 Raman spectra were recorded from 38 snap-frozen prostate samples collected at transurethral resection of the prostate. The spectra were correlated with the histologic features and used to construct separate diagnostic algorithms for the bladder and prostate. These algorithms were tested as to their ability to determine the pathologic finding of a sample from its Raman spectrum. Results. The bladder algorithm was able to differentiate benign samples (normal and cystitis) from malignant samples (transitional cell carcinoma), with an overall accuracy of 84%. The prostate algorithm was able to differentiate benign samples (benign prostatic hyperplasia and prostatitis) from malignant samples (prostate cancer), with an overall accuracy of 86%. Conclusions. The results of this study have demonstrated that the clinical Raman system can provide an accurate and objective method to diagnose prostate and bladder cancer in vitro. Because the Raman probe is suitable for use during endoscopic, laparoscopic, or open procedures, this work paves the way for in vivo studies.
引用
收藏
页码:1126 / 1130
页数:5
相关论文
共 21 条
[1]  
[Anonymous], 1998, Chemometrics: A Practical Guide
[2]   The use of Raman spectroscopy to identify and characterize transitional cell carcinoma in vitro [J].
Crow, P ;
Uff, JS ;
Farmer, JA ;
Wright, MP ;
Stone, N .
BJU INTERNATIONAL, 2004, 93 (09) :1232-1236
[3]   Optical diagnostics in urology: current applications and future prospects [J].
Crow, P ;
Stone, N ;
Kendall, CA ;
Persad, RA ;
Wright, MPJ .
BJU INTERNATIONAL, 2003, 92 (04) :400-407
[4]   The use of Raman spectroscopy to identify and grade prostatic adenocarcinoma in vitro [J].
Crow, P ;
Stone, N ;
Kendall, CA ;
Uff, JS ;
Farmer, JAM ;
Barr, H ;
Wright, MPJ .
BRITISH JOURNAL OF CANCER, 2003, 89 (01) :106-108
[5]  
Flannery B.P., 1992, NUMERICAL RECIPES C
[6]  
Jackson JE, 1991, A user's guide to principal component analysis
[7]  
MAHADEVAN A, 1995, P SOC PHOTO-OPT INS, V2388, P110, DOI 10.1117/12.208469
[8]   Near-infrared Raman spectroscopy for in vitro detection of cervical precancers [J].
Mahadevan-Jansen, A ;
Mitchell, MF ;
Ramanujam, N ;
Malpica, A ;
Thomsen, S ;
Utzinger, U ;
Richards-Kortum, R .
PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1998, 68 (01) :123-132
[9]   Diagnostic potential of near-infrared Raman spectroscopy in the colon: differentiating adenomatous from hyperplastic polyps [J].
Molckovsky, A ;
Song, LMWK ;
Shim, MG ;
Marcon, NE ;
Wilson, BC .
GASTROINTESTINAL ENDOSCOPY, 2003, 57 (03) :396-402
[10]  
Otto M., 1999, CHEMOMETRICS STAT CO