Cross-talk between human mesenchymal stem/progenitor cells (MSCs) and rat hippocampal slices in LPS-stimulated cocultures: the MSCs are activated to secrete prostaglandin E2

被引：27

作者：

Foraker, Jessica E. ^{[1
]}

Oh, Joo Youn ^{[1
]}

Ylostalo, Joni H. ^{[1
]}

Lee, Ryang Hwa ^{[1
]}

Watanabe, Jun ^{[1
]}

Prockop, Darwin J. ^{[1
]}

机构：

[1] Texas A&M Hlth Sci Ctr, Coll Med Scott & White, Inst Regenerat Med, Temple, TX 76502 USA

来源：

JOURNAL OF NEUROCHEMISTRY | 2011年 / 119卷 / 05期

关键词：

cyclo-oxygenase-2; inflammation; lipopolysaccharide; OHSC; organotypic; PGE2; MARROW STROMAL CELLS; STEM-CELLS; NEURODEGENERATIVE DISEASES; CYTOKINE PRODUCTION; IMMUNE FUNCTION; BRAIN; MICROGLIA; CULTURES; NEUROPROTECTION; ASTROCYTES;

D O I：

10.1111/j.1471-4159.2011.07511.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Mesenchymal stem/progenitor cells (MSCs) improve functional outcome in a number of disease models through suppression of inflammation. However, their effects on neuroinflammation are unknown. In this study, we show that MSCs suppress endotoxin-induced glial activation in organotypic hippocampal slice cultures (OHSCs). Lipopolysaccharide-stimulated OHSCs activated MSCs to increase the expression of cyclo-oxygenase-2 and produce prostaglandin E2. MSC-derived prostaglandin E2, then suppressed pro-inflammatory cytokine production by the OHSCs. Together, the results suggest the potential anti-inflammatory mechanism of MSCs in models of disease and support earlier observations that MSCs may offer a therapy for neuroinflammation produced by trauma or disease.

引用

页码：1052 / 1063

页数：12

共 35 条

[1]

Human mesenchymal stem cells modulate allogeneic immune cell responses [J].

Aggarwal, S ;

Pittenger, MF .

BLOOD, 2005, 105 (04) :1815-1822

[2]

Immune function of microglia [J].

Aloisi, F .

GLIA, 2001, 36 (02) :165-179

[3]

Aloisi F, 1999, J NEUROSCI RES, V56, P571

[4]

Prostaglandins and other lipid mediators in Alzheimer's disease [J].

Bazan, NG ;

Colangelo, V ;

Lukiw, WJ .

PROSTAGLANDINS & OTHER LIPID MEDIATORS, 2002, 68-9 :197-210

[5]

Ballistic labeling and dynamic imaging of astrocytes in orcranotypic hippocampal slice cultures [J].

Benediktsson, AM ;

Schachtele, SJ ;

Green, SH ;

Dailey, ME .

JOURNAL OF NEUROSCIENCE METHODS, 2005, 141 (01) :41-53

[6]

Cyclooxygenase 2 (COX-2) inhibition increases the inflammatory response in the brain during systemic immune stimuli [J].

Blais, V ;

Turrin, NP ;

Rivest, S .

JOURNAL OF NEUROCHEMISTRY, 2005, 95 (06) :1563-1574

[7]

Microglia-mediated neurotoxicity: uncovering the molecular mechanisms [J].

Block, Michelle L. ;

Zecca, Luigi ;

Hong, Jau-Shyong .

NATURE REVIEWS NEUROSCIENCE, 2007, 8 (01) :57-69

[8]

The MSC: An Injury Drugstore [J].

Caplan, Arnold I. ;

Correa, Diego .

CELL STEM CELL, 2011, 9 (01) :11-15

[9]

Cyclooxygenases-1 and-2 differentially modulate leukocyte recruitment into the inflamed brain [J].

Choi, S-H ;

Aid, S. ;

Choi, U. ;

Bosetti, F. .

PHARMACOGENOMICS JOURNAL, 2010, 10 (05) :448-457

[10]

Rapid expansion of recycling stem cells in cultures of plastic-adherent cells from human bone marrow [J].

Colter, DC ;

Class, R ;

DiGirolamo, CM ;

Prockop, DJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (07) :3213-3218

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