Osteoinduction in human fat-derived stem cells by recombinant human bone morphogenetic protein-2 produced in Escherichia coli

被引：29

作者：

Bessa, Paulo C.

Pedro, A. J.

Kloesch, B.

Nobre, A.

van Griensven, M.

Reis, R. L.

Casal, M.

机构：

[1] Univ Minho, Dept Biol, CBMA Mol & Environm Biol Ctr, P-4710057 Braga, Portugal

[2] Univ Minho, Dept Polymer Engn, 3Bs Res Grp Biomat Biodegradables & Biomimet, P-4710057 Braga, Portugal

[3] IBB, PT Govt Associated Lab, P-4710057 Braga, Portugal

[4] Ludwig Boltzmann Inst Expt & Clin Traumatol, A-1200 Vienna, Austria

来源：

BIOTECHNOLOGY LETTERS | 2008年 / 30卷 / 01期

关键词：

bone morphogenetic protein-2; recombinant protein purification; stem cells; tissue engineering;

D O I：

10.1007/s10529-007-9501-8

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Bioactive recombinant human bone morphogenetic protein-2 (rhBMP-2) was obtained using Escherichia coli pET-25b expression system: 55 mg purified rhBMP-2 were achieved per g cell dry wt, with up to 95% purity. In murine C2C12 cell line, rhBMP-2 induced an increase in the transcription of Smads and of osteogenic markers Runx2/Cbfa1 and Osterix, measured by semi-quantitative RT-PCR. Bioassays performed in human fat-derived stem cells showed an increased activity of the early osteogenic marker, alkaline phosphatase, and the absence of cytotoxicity.

引用

页码：15 / 21

页数：7

共 31 条

[1] Bone induction by Escherichia coli-derived recombinant human bone morphogenetic protein-2 compared with Chinese hamster ovary cell-derived recombinant human bone morphogenetic protein-2 [J].